Possible Regulation of LDL-Receptor by Naringenin in Hepg2 Hepatoma Cell Line
Bibliographic Data
| ID | 20346774 |
|---|---|
| Authors | Nora A Bawazeer (Taif University), Hani Choudary (King Abdulaziz University), Mazin A Zamzami (0000-0002-6709-3227, King Abdulaziz University), Wesam H Abdulaal (0000-0002-2510-1946, King Abdulaziz University), Mustafa Zeyadi (0000-0002-6138-0102, King Abdulaziz University), Ashwaq Albukhari (King Abdulaziz University), Bruce Middleton (University of Nottingham), Said S Moselhy (0000-0002-2384-3700, Ain Shams University) |
| Year | 2016 |
| Volume | 14 |
| Issue | 1 |
| Pages | 278-287 |
| Publication date | 2016-12-23 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) (PUBLISHER) |
| DOI | 10.21010/ajtcam.v14i1.30 |
| PMID | 28480406 |
| OpenAlex | W2553936332 |
| Language | EN |
BACKGROUND: High plasma concentration of low-density lipoprotein cholesterol (LDL-c) plays a significant role in the incidence of atherosclerosis and coronary heart diseases (CHD). MATERIALS AND METHODS: The purpose of this study was to investigate the mechanism by which citrus flavonoids, naringenin regulate the LDL receptor (LDLr) gene in human liver using the human hepatoma cell line, HepG2 as a model. RESULTS: Time-course transient transfection of HepG2 cells with luciferase reporter-gene constructs incorporating the promoters of SREBP-1a,-1c, -2 and LDLr, revealed that in lipoprotein-deficient medium (LPDM), only SREBP-1a promoter activity was increased significantly after 4h exposure to 200μM naringenin respectively. However, after 24h incubation with 200μM naringenin the gene expression activities of all the SREBP-1a, -1c, -2 and LDLr promoter-constructs were increased significantly. The effects of both 200μM naringenin on elevating LDLr mRNA are possibly due to regulation of gene transcription by SREBP-la and SREBP-2. However, the suppression effect of 200μM naringenin on hepatic SREBP-1c mRNA expression is likely associated with the reduction in mRNA expression of both acetyl-CoA carboxylase and fatty acid synthase in human hepatoma HepG2 cells. It was found that, 200μM naringenin was likely to stimulate LDLr gene expression via increase phosphorylation of PI3K and ERK1/2 which enhance the transcription factors SREBP-1a and SREBP-2 mRNA levels and increased their protein maturation in human hepatoma HepG2 cell. CONCLUSION: Diets supplemented with naringenin could effectively reduce mortality and morbidity from coronary heart diseases and as cardio-protective effects in humans
Biology · Cholesterol · Fatty acid synthase · Flavonoid · Gene · Gene expression · LDL receptor · Lipid metabolism · Lipoprotein · Naringenin · Promoter · Sterol regulatory element-binding protein · Cancer, Lipids, and Metabolism · Chemistry · Cholesterol and Lipid Metabolism · Medicine · Peroxisome Proliferator-Activated Receptors · Biochemistry · Endocrinology · Internal Medicine · Molecular Biology
| Highly cited | No |
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