A Study of Sperm DNA Damage Mechanism Based on miRNA Sequencing
Bibliographic Data
To analyze the differential expression profiles of microRNAs (miRNAs) in spermatozoa of patients with sperm DNA damage and to investigate the role of miRNAs in sperm DNA damage. Male infertility patients with sperm DNA damage who attended the First Affiliated Hospital of Henan University of Chinese Medicine from October 2023 to December 2023 were selected and included in this study as a case group. Fertile healthy men who were seen at the health check-up center during the same period and diagnosed by examination were also included as a control group. Sperm miRNA expression was detected in patients with sperm DNA damage (case group, n = 5) and healthy medical check-ups (control group, n = 5) using high-throughput sequencing technology. The differentially expressed miRNAs between the two groups were bioinformatically analyzed to explore the main biological functions of the target genes. We found that 63 miRNAs were significantly changed in the spermatozoa of patients with sperm DNA damage,|log2 (foldchange)| ≥ 1, p
Andrology · Bioinformatics · Biology · DNA · DNA damage · Gene · Gene expression · Gene ontology · Infertility · KEGG · Male infertility · microRNA · Protein kinase B · Signal transduction · Sperm · Medicine · MicroRNA in disease regulation · Selenium in Biological Systems · Sperm and Testicular Function · Genetics
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| Highly cited | No |