Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs

Dose – Response Relationship

Bibliographic Data

ID22070796
AuthorsRenata Sisto (0000-0002-5086-7003, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro, corresponding author), Delia Cavallo (0000-0002-2331-1464, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Cinzia Lucia Ursini (0000-0003-3577-0331, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Anna Maria Fresegna (Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Aureliano Ciervo (Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Raffaele Maiello (0000-0003-2339-7082, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Enrico Paci (0000-0003-3448-9345, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Daniela Pigini (0000-0002-7582-6179, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Monica Gherardi (0000-0003-0786-8470, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Andrea Gordiani (Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Nunziata L’Episcopo (Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Nunziata L'Episcopo, Giovanna Tranfo (0000-0001-8973-6046, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Pasquale Capone (0000-0002-2553-0345, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Damiano Carbonari (Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro), Barbara Balzani, Pieranna Chiarella (0000-0001-8367-4032, Istituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro)
Year2020
Volume8
Pages445-445
Publication date2020-08-19
Open AccessYes
TypeARTICLE
VenueFrontiers in Public Health (JOURNAL)
Journal identifiersISSN: 2296-2565 • E-ISSN: 2296-2565
PublisherFrontiers Media SA (PUBLISHER • CH)
DOI10.3389/fpubh.2020.00445
PMID32974263
OpenAlexW3049977186
LanguageEN
Citations received2
References cited35

Volatile organic compounds (VOCs) are present in several working activities. This work is aimed at comparing oxidative stress and DNA damage biomarkers to specific VOCs in the occupational exposure of painters. Dose-response relationships between biomarkers of oxidative stress and of dose were studied. Unmetabolized VOCs and their urinary metabolites were analyzed. Urinary Methylhyppuric acids (MHIPPs, xylenes metabolite), Phenylglyoxylic and Mandelic acid (PGA, MA ethylbenzene metabolites), S-Benzylmercapturic acid (SBMA, toluene metabolite), and S-Phenylmercapturic acid (SPMA, benzene metabolite) were quantified at the end of work-shift. Oxidative stress was determined by: urinary excretion of 8-oxodGuo, 8-oxoGua and 8-oxoGuo and direct/oxidative DNA damage in blood by Fpg-Comet assay. Multivariate linear regression models were used to assess statistical significance of the association between dose and effect biomarkers. The regressions were studied with and without the effect of hOGG1 and XRCC1 gene polymorphisms. Statistically significant associations were found between MHIPPs and both 8-oxoGuo and oxidative DNA damage effect biomarkers measured with the Comet assay. Oxidative DNA damage results significantly associated with airborne xylenes and toluene, whilst 8-oxodGuo was significantly related to urinary xylenes and toluene. Direct DNA damage was significantly associated to SBMA. XRCC1 wild-type gene polymorphism was significantly associated with lower oxidative and total DNA damage with respect to heterozygous and mutant genotypes. The interpretation of the results requires some caution, as the different VOCs are all simultaneously present in the mixture and correlated among them

Comet Assay · DNA · DNA damage · Metabolite · Muconic acid · Oxidative stress · Urine · Air Quality and Health Impacts · Carcinogens and Genotoxicity Assessment · Chemistry · Indoor Air Quality and Microbial Exposure · Biochemistry · Environmental Chemistry · Food Science

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