Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia
Bibliographic Data
| ID | 22075485 |
|---|---|
| Authors | Yibeltal Assefa Atalay (0009-0009-3252-8161, Wolaita Sodo University, corresponding author), Embialle Mengistie (Hawassa University), Alemu Tolcha (Hawassa University), Belete Birhan (0009-0005-3010-4366, Wolaita Sodo University), Getachew Asmare Adella (0000-0001-8469-7754, Wolaita Sodo University), Getachew Asmare, Natnael Atnafu Gebeyehu (0000-0001-9583-833X, Wolaita Sodo University), Kelemu Abebe Gelaw (0000-0001-7157-4616, Wolaita Sodo University) |
| Year | 2023 |
| Volume | 11 |
| Pages | 1194850-1194850 |
| Publication date | 2023-11-02 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Frontiers in Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2296-2565 • E-ISSN: 2296-2565 |
| Publisher | Frontiers Media SA (PUBLISHER • CH) |
| DOI | 10.3389/fpubh.2023.1194850 |
| PMID | 38026319 |
| OpenAlex | W4388328201 |
| Language | EN |
| References cited | 27 |
Background Air is the agent of pathogenic microbes that cause significant problems in the hospital environment. Multidrug resistance poses a major therapeutic challenge to these airborne microorganisms in hospital indoor environments. Method and materials This study was conducted at Adare General Hospital in Hawassa City, Sidama, Ethiopia. A cross-sectional study was conducted. The proportional allocation method was used to select the sampled 50 rooms from the total available rooms in each category of wards and staff offices. A total of 100 indoor air samples were collected using settle plates in all units twice a day, morning (9:00–4:00 a.m.) and afternoon (3:00–4:00 p.m.). The types and number of colonies were determined in the laboratory, and the pathogenic bacteria were isolated by appropriate bacteriological techniques. Antimicrobial susceptibility testing was performed on Mueller-Hinton agar for each potentially pathogenic bacterium isolated. For each bacterium, a total of 12 antibiotics were tested using the Kirby-Bauer disk diffusion method. The test organism was adjusted to McFarland turbidity standards of 0.5. Data were entered and analyzed using the SPSS version 25 window. Descriptive analysis and one-way analysis of variance were performed. Results The indoor air bacterial load of Adare General Hospital was found in the range between 210 and 3,224 CFU/m 3 . The highest indoor air bacterial load was identified from the gynecology ward with a mean of 2,542.5CFU/m 3 at p < 0.05. From 100 indoor air samples, a total of 116 bacterial pathogen isolates were obtained. Gram-positive isolates predominated at 72.4%, of which 37.1% were Staphylococcus aureus , 26.7% were coagulase-negative Staphylococci , and the rest 8.6% were Streptococcus pyogenes . The isolation of pathogenic bacteria Staphylococcus aureus and coagulase-negative Staphylococci showed a high level of resistance to ampicillin. Conclusion A high bacterial load was found in the study area as compared to different indoor air biological standards. Staphylococcus aureus and coagulase-negative Staphylococci were the isolated predominant bacteria. Attention should be given to preventing and minimizing those environmental factors that favor the multiplication of bacteria in the indoor environment of a hospital for the safe health of patients, visitors, and staff
Agar diffusion test · Antibacterial activity · Antibiotic resistance · Antibiotics · Bacteria · Biology · General hospital · Indoor air · Pathogenic bacteria · Veterinary medicine · Antimicrobial agents and applications · Environmental Science · Indoor Air Quality and Microbial Exposure · Infection Control in Healthcare · Medicine · Environmental Engineering · Microbiology · Pediatrics
| Citation velocity | historical |
|---|---|
| Highly cited | No |