Prevalence of Antibiotic Resistome in Ready-to-Eat Salad
Bibliographic Data
| ID | 22079477 |
|---|---|
| Authors | Shu-Yi-Dan Zhou, Shu‐Yi‐Dan Zhou (0000-0002-2585-7305, University of Chinese Academy of Sciences), Meng-Yun Wei (Chinese Academy of Sciences), Madeline Giles (0000-0001-7582-2867, James Hutton Institute), Roy Neilson (0000-0003-4544-2227, James Hutton Institute), Fei Zheng (0000-0003-3513-4255, University of Chinese Academy of Sciences), Qi Zhang (0009-0000-5229-2496, Zhejiang University of Technology), Yong‐Guan Zhu (0000-0003-3861-8482, University of Chinese Academy of Sciences), Yong-Guan Zhu, Xiao-Ru Yang, Xiao‐Ru Yang (0000-0001-8064-4639, Chinese Academy of Sciences, corresponding author) |
| Year | 2020 |
| Volume | 8 |
| Pages | 92-92 |
| Publication date | 2020-03-25 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Frontiers in Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2296-2565 • E-ISSN: 2296-2565 |
| Publisher | Frontiers Media SA (PUBLISHER • CH) |
| DOI | 10.3389/fpubh.2020.00092 |
| PMID | 32269985 |
| OpenAlex | W3013746296 |
| Language | EN |
| Citations received | 2 |
| References cited | 65 |
Ready-to-eat salad harbors microorganisms that may carry various antibiotic resistance genes (ARGs). However, few studies have focused on the prevalence of ARGs on salad, thus underestimating the risk of ARGs transferring from salad to consumers. In this small-scale study, high-throughput quantitative PCR was used to explore the presence, prevalence and abundance of ARGs associated with serving salad sourced from two restaurant types, fast-food chain and independent casual dining. A total of 156 unique ARGs and 9 mobile genetic elements (MGEs) were detected on the salad items assessed. The abundance of ARGs and MGEs were significantly higher in independent casual dining than fast-food chain restaurants. Absolute copies of ARGs in salad were 1.34×107 to 2.71×108 and 1.90×108 to 4.87 ×108 copies per g salad in fast-food and casual dining restaurants, respectively. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the dominant bacterial phyla detected from salad samples. Pseudomonas, Acinetobacter, Exiguobacterium, Weissella, Enterobacter, Leuconostoc, Pantoea, Serratia, Erwinia and Ewingella were the ten most dominant bacterial genera found in salad samples. A significant positive correlation between ARGs and MGEs was detected. These results integrate knowledge about the ARGs in ready-to-eat salad and highlight the potential impact of ARGs transfer to consumers
16S ribosomal RNA · Antibiotic resistance · Antibiotics · Bacteria · Bacterial phyla · Bacteroidetes · Biology · Enterobacter · Escherichia coli · Fermentation · Firmicutes · Lactobacillus · Leuconostoc · Pantoea · Proteobacteria · Pseudomonas · Resistome · Weissella · Antibiotic Resistance in Bacteria · Biosensors and Analytical Detection · Pharmaceutical and Antibiotic Environmental Impacts · Food Science · Genetics · Microbiology
| Citations per year | 0.5 |
|---|---|
| Citation span | 2022 - 2025 (4) |
| Highly cited | No |