Metagenomic Next-Generation Sequencing vs. Traditional Microbiological Tests for Diagnosing Varicella-Zoster Virus Central Nervous System Infection
Bibliographic Data
| ID | 22081833 |
|---|---|
| Authors | Yunqi Zhu (0009-0006-0981-0191, Nanjing University of Chinese Medicine), Miaomiao Xu (0000-0001-8544-6754, Nanjing University of Chinese Medicine), Chengyuan Ding (0009-0001-6328-7083, Nanjing Medical University), Zhihang Peng (0000-0003-4372-2487, Nanjing Medical University), Weixiao Wang (0000-0003-1485-5002, Nanjing University of Chinese Medicine), Binghu Sun (Nanjing University of Chinese Medicine), Jian Cheng (0000-0002-3755-2517, Nanjing University of Chinese Medicine), Chen Chen (0000-0002-9786-8003, Nanjing University of Chinese Medicine), Wei Chen (0000-0002-9419-148X, Nanjing University of Chinese Medicine), Hongxia Wei (0009-0008-2153-9132, Nanjing University of Chinese Medicine, corresponding author), Zhiliang Hu (0000-0001-8543-821X, Nanjing University of Chinese Medicine, corresponding author) |
| Year | 2022 |
| Volume | 9 |
| Pages | 738412-738412 |
| Publication date | 2022-01-21 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Frontiers in Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2296-2565 • E-ISSN: 2296-2565 |
| Publisher | Frontiers Media SA (PUBLISHER • CH) |
| DOI | 10.3389/fpubh.2021.738412 |
| PMID | 35127608 |
| OpenAlex | W4205949695 |
| Language | EN |
| Citations received | 1 |
| References cited | 34 |
Background Unbiased metagenomic next-generation sequencing (mNGS) detects pathogens in a target-independent manner. It is not well-understood whether mNGS has comparable sensitivity to target-dependent nucleic acid test for pathogen identification. Methods This study included 31 patients with chickenpox and neurological symptoms for screening of possible varicella-zoster virus (VZV) central nervous system (CNS) infection. Microbiological diagnosing of VZV cerebrospinal fluid (CSF) infection was performed on stored CSF samples using mNGS, quantitative and qualitative VZV-specific PCR assays, and VZV IgM antibodies test. Results The median age was 30.0 [interquartile range (IQR), 24.3–33.3] years. 51.6% of the patients were men. About 80.6% of the patients had normal CSF white blood cell counts (≤ 5 × 10 6 /L). VZV IgM antibodies presented in 16.1% of the CSF samples, and nucleic acids were detectable in 16.1 and 9.7% using two different VZV-specific real-time PCR protocols. Intriguingly, maximal identification of VZV elements was achieved by CSF mNGS ( p = 0.001 and p = 007; compared with qualitative PCR and VZV IgM antibody test, respectively), with sequence reads of VZV being reported in 51.6% (16/31) of the CSF samples. All VZV PCR positive samples were positive when analyzed by mNGS. Of note, human betaherpesvirus 6A with clinical significance was unexpectedly detected in one CSF sample. Conclusions Our study suggests that CSF mNGS may have higher sensitivity for VZV detection than CSF VZV PCR and antibody tests, and has the advantage of identifying unexpected pathogens
Antibody · Biology · Cerebrospinal fluid · Chickenpox · Disease · Nucleic acid test · Pathogen · Pathology · Varicella zoster virus · Virus · Bacterial Infections and Vaccines · Herpesvirus Infections and Treatments · Medicine · Multiple Sclerosis Research Studies · Immunology · Virology
| Citations per year | 0.33 |
|---|---|
| Citation span | 2023 - 2023 (1) |
| Highly cited | No |