Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit
Bibliographic Data
| ID | 22089753 |
|---|---|
| Authors | Meijin Li, Mei‐Jin Li (0000-0002-8245-5556, Guangdong Province Women and Children Hospital), Jing Wang (0000-0002-7594-8539), Zhongwei Yao (Guangdong Province Women and Children Hospital), Hailing Liao (Guangdong Province Women and Children Hospital), Shu‐Fen Su (0000-0001-9626-3101, Guangdong Province Women and Children Hospital), Shufen Su, Xuying Yang, Mingzhou Xie, Yi-nan Zheng (Guangdong Province Women and Children Hospital, corresponding author), Yinan Zheng (0000-0002-2006-7320) |
| Year | 2023 |
| Volume | 11 |
| Pages | 1177069-1177069 |
| Publication date | 2023-06-15 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Frontiers in Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2296-2565 • E-ISSN: 2296-2565 |
| Publisher | Frontiers Media SA (PUBLISHER • CH) |
| DOI | 10.3389/fpubh.2023.1177069 |
| PMID | 37397737 |
| OpenAlex | W4380680577 |
| Language | EN |
| Citations received | 1 |
| References cited | 56 |
Background: Pneumonia is a significant cause of morbidity and mortality in children. Metagenomic next-generation sequencing (mNGS) has the potential to assess the landscape of pathogens responsible for severe pulmonary infection. Methods: Bronchoalveolar lavage fluid (BALF) samples of 262 children with suspected pulmonary infections were collected from April 2019 to October 2021 in the Pediatric Intensive Care Unit (PICU) of Guangdong Women and Children Hospital. Both mNGS and conventional tests were utilized for pathogen detection. Results: A total of 80 underlying pathogens were identified using both mNGS and conventional tests. Respiratory syncytial virus (RSV), Staphylococcus aureus and rhinovirus were the most frequently detected pathogens in this cohort. The incidence rate of co-infection was high (58.96%, 148/251), with bacterial-viral agents most co-detected. RSV was the main pathogen in children younger than 6 months of age, and was also commonly found in older pediatric patients. Rhinovirus was prevalent in children older than 6 months. Adenovirus and Mycoplasma pneumoniae were more prevalent in children older than 3 years than in other age groups. Pneumocystis jirovecii was detected in nearly 15% of children younger than 6 months. Besides, influenza virus and adenovirus were rarely found in 2020 and 2021. Conclusions: Our study highlights the importance of using advanced diagnostic techniques like mNGS to improve our understanding of the microbial epidemiology of severe pneumonia in pediatric patients
Cohort · Intensive care medicine · Intensive care unit · Mycoplasma pneumoniae · Pathogen · Pediatric intensive care unit · Pneumonia · Rhinovirus · Virus · Medicine · Pneumocystis jirovecii pneumonia detection and treatment · Pneumonia and Respiratory Infections · Respiratory viral infections research · Epidemiology · Immunology · Internal Medicine · Pediatrics
| Citations per year | 0.5 |
|---|---|
| Citation span | 2024 - 2024 (1) |
| Highly cited | No |