Applications of VirScan to broad serological profiling of bat reservoirs for emerging zoonoses
Bibliographic Data
| ID | 22090708 |
|---|---|
| Authors | Emily Cornelius Ruhs (0000-0002-0546-6593, Field Museum of Natural History, corresponding author), Wan Ni Chia (0000-0001-7287-694X, Duke-NUS Medical School), Randy Foo (0000-0001-9661-8662, Duke-NUS Medical School), Alison J Peel (0000-0003-3538-3550, Griffith University), Yimei Li (0000-0001-9067-6992, Princeton University), H Benjamin Larman (0000-0001-7863-196X, Johns Hopkins University), Aaron T Irving (0000-0002-0196-1570, Blood Center of Zhejiang Province), Lin‐Fa Wang (0000-0003-2752-0535, SingHealth), Linfa Wang, Cara E Brook (0000-0003-4276-073X, University of Chicago) |
| Year | 2023 |
| Volume | 11 |
| Pages | 1212018-1212018 |
| Publication date | 2023-09-22 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Frontiers in Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2296-2565 • E-ISSN: 2296-2565 |
| Publisher | Frontiers Media SA (PUBLISHER • CH) |
| DOI | 10.3389/fpubh.2023.1212018 |
| PMID | 37808979 |
| OpenAlex | W4386960364 |
| Language | EN |
| References cited | 74 |
Introduction: Bats are important providers of ecosystem services such as pollination, seed dispersal, and insect control but also act as natural reservoirs for virulent zoonotic viruses. Bats host multiple viruses that cause life-threatening pathology in other animals and humans but, themselves, experience limited pathological disease from infection. Despite bats' importance as reservoirs for several zoonotic viruses, we know little about the broader viral diversity that they host. Bat virus surveillance efforts are challenged by difficulties of field capture and the limited scope of targeted PCR- or ELISA-based molecular and serological detection. Additionally, virus shedding is often transient, thus also limiting insights gained from nucleic acid testing of field specimens. Phage ImmunoPrecipitation Sequencing (PhIP-Seq), a broad serological tool used previously to comprehensively profile viral exposure history in humans, offers an exciting prospect for viral surveillance efforts in wildlife, including bats. Methods: Here, for the first time, we apply PhIP-Seq technology to bat serum, using a viral peptide library originally designed to simultaneously assay exposures to the entire human virome. Results: . Consistent with results from humans, we find that both total peptide hits (the number of enriched viral peptides in our library) and the corresponding number of inferred past virus exposures in bat hosts were correlated with poor bat body condition scores and increased with age. High and low body condition scores were associated with either seropositive or seronegative status for different viruses, though in general, virus-specific age-seroprevalence curves defied assumptions of lifelong immunizing infection, suggesting that many bat viruses may circulate via complex transmission dynamics. Discussion: Overall, our work emphasizes the utility of applying biomedical tools, like PhIP-Seq, first developed for humans to viral surveillance efforts in wildlife, while highlighting opportunities for taxon-specific improvements
Antibody · Biology · Ebola virus · Gene · Hendra Virus · Human virome · Metagenomics · Natural reservoir · Serology · Virus · Zoology · Rabies epidemiology and control · Virology and Viral Diseases · Zoonotic diseases and public health · Genetics · Immunology · Virology
| Citation velocity | historical |
|---|---|
| Highly cited | No |