Identification and Quantification of Related Substances of Vitamind3 in Its Prepartions by Rp-HPLC
Bibliographic Data
| ID | 22198227 |
|---|---|
| Authors | Syed Wadood Ali Shah (0000-0001-8143-2363, Vels University), Syed Shah, Archana M, M Archana (0000-0001-7264-2182, Vels University) |
| Year | 2024 |
| Volume | 5 |
| Issue | 5 |
| Publication date | 2024-05-31 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | ShodhKosh: Journal of Visual and Performing Arts (JOURNAL) |
| Journal identifiers | ISSN: 2582-7472 • E-ISSN: 2582-7472 |
| Publisher | Granthaalayah Publications and Printers (PUBLISHER • IN) |
| DOI | 10.29121/shodhkosh.v5.i5.2024.2782 |
| OpenAlex | W4404511489 |
| Language | EN |
| References cited | 14 |
An analytical approach for quantifying vitamin D3 related substancesmethod was developed by using high-performance liquid chromatography (HPLC). The separation was achieved using a hypersil Silica, 250mm x 4.6mm, 5μm column, and a mobile phase composition of n-pentanol and n-hexane was used. The wavelength of the chromatogram was 265 nm at a temperature of 20°C, the flow rate was 2.0 mL/min. The method was specific, and linear within the range of 50%-150% and has demonstrated good recovery, inter day and intraday precision. As well as robustness, the detection and quantification limits were found to be within the limits at .001 and 0.0037 g/ml, respectively. The developed method ismore precise, accurate, specific and robust to determine the related substances of Vitamin D3 in its preparations and validated according to ICH guidelines Q2(R1)
Biology · Chromatography · High-performance liquid chromatography · Chemistry · Vitamin C and Antioxidants Research · Vitamin D Research Studies · Vitamin K Research Studies
| Highly cited | No |
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