Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina
Bibliographic Data
| ID | 23451803 |
|---|---|
| Authors | Thanh Hoang, Ashley Wright Baker (corresponding author), Dong Won Kim (0000-0001-8624-0168), Haley Appel, Nicole A Pannullo, Patrick Leavey, Manabu Ozawa, Sika Zheng, Minzhong Yu, Neal S Peachey, Seth Blackshaw (0000-0002-1338-8476) |
| Year | 2022 |
| Volume | 39 |
| Issue | 11 |
| Pages | 110849 |
| Publication date | 2022-06-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Cell Reports (JOURNAL) |
| Journal identifiers | ISSN: 2211-1247 • E-ISSN: 2639-1856 |
| Publisher | Elsevier BV (PUBLISHER) |
| DOI | 10.1016/j.celrep.2022.110849 |
| OpenAlex | W35705053 |
| Language | EN |
| Citations received | 2 |
| References cited | 30 |
Direct reprogramming of glia into neurons is a potentially promising approach for the replacement of neurons lost to injury or neurodegenerative disorders. Knockdown of the polypyrimidine tract-binding protein Ptbp1 has been recently reported to induce efficient conversion of retinal Mϋller glia into functional neurons. Here, we use a combination of genetic lineage tracing, single-cell RNA sequencing (scRNA-seq), and electroretinogram analysis to show that selective induction of either heterozygous or homozygous loss-of-function mutants of Ptbp1 in adult retinal Mϋller glia does not lead to any detectable level of neuronal conversion. Only a few changes in gene expression are observed in Mϋller glia following Ptbp1 deletion, and glial identity is maintained. These findings highlight the importance of using genetic manipulation and lineage-tracing methods in studying cell-type conversion.
Art · Convict · Epistemology · Genealogy · Literature · Objectivity (philosophy) · Political science · Sociology · Australian History and Society · Commonwealth, Australian Politics and Federalism · History · Law · Philosophy
| Citations per year | 0.05 |
|---|---|
| Citation span | 1985 - 1992 (8) |
| Highly cited | No |