Protection Mechanism of Metal Ion Pre-Stress on Lactobacillus acidophilus CICC 6074 under Acid Tolerance
Bibliographic Data
| ID | 23539584 |
|---|---|
| Authors | Tao Zhang (0000-0002-4890-1671, Ningbo University), Yuxing Guo (0000-0002-7656-3470, Nanjing Normal University), Xiankang Fan (Ningbo University), Mingzhen Liu (Ningbo University), Jue Xu (0000-0002-9033-9619, Ningbo University), Xiaoqun Zeng (0000-0002-4599-2010, Ningbo University), Yangying Sun (Ningbo University), Zhen Wu (0000-0003-3655-1069, Ningbo University), Daodong Pan (0000-0003-2935-9781, Ningbo University) |
| Year | 2023 |
| Volume | 71 |
| Issue | 36 |
| Pages | 13304-13315 |
| Publication date | 2023-09-13 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Journal of Agricultural and Food Chemistry (JOURNAL) |
| Journal identifiers | ISSN: 0021-8561 • E-ISSN: 1520-5118 |
| Publisher | American Chemical Society (ACS) (PUBLISHER) |
| DOI | 10.1021/acs.jafc.3c01970 |
| OpenAlex | W37639527 |
| Language | EN |
| Citations received | 2 |
| References cited | 50 |
The prerequisite for the probiotic effect of lactic acid bacteria is that they could survive the acid stress environment of production and application. In this experiment, the mechanism for the effect of different metal ion pre-stress on the acid-tolerant survival of Lactobacillus was investigated. Scanning electron microscopy, Fourier infrared spectroscopy, and flow cytometry were used to analyze the condition of bacteria after acid treatment, which revealed that different metal ion pre-stress could improve the survival ability of Lactobacillus acidophilus CICC 6074 under low acid conditions by improving cell morphology, mitigating cell membrane damage, and regulating surface protein expression. Furthermore, Tandem Mass Tags (TMT) proteomic analysis revealed that Mn 2+ pre-stress showed relatively more superior protective effects on acid tolerance in L. acidophilus CICC 6074 through activation of DNA replication, RNA synthesis, S-layer protein secretion, H + -ATPase enzyme activity, etc. This study will provide new ideas and a theoretical basis for the development and application of lactic acid bacteria.
Developmental psychology · Physics · Primary (astronomy) · Digital Storytelling and Education · Educator Training and Historical Pedagogy · Psychology
| Citations per year | 0.06 |
|---|---|
| Citation span | 1993 - 2000 (8) |
| Highly cited | No |