DNA extraction from sternum bone for identification of a saponified body
Use of a Modified Protocol
Bibliographic Data
| ID | 4913355 |
|---|---|
| Authors | Antonio De Donno (0000-0002-4238-2495, University of Bari Aldo Moro), Federica Mele (0000-0003-4289-8907, University of Bari Aldo Moro), Stefania Lonero Baldassarra (0000-0002-9971-3240, University of Bari Aldo Moro), Alessandra Martini, Alessandra Cadete Martini (0000-0002-8447-6756, University of Bari Aldo Moro), Chiara Lauretti (University of Bari Aldo Moro), Matteo Favia (0000-0001-9623-2268, University of Bari Aldo Moro), Francesco Introna (0000-0002-8219-5467, University of Bari Aldo Moro), Valeria Santoro (0000-0002-1612-8151, University of Bari Aldo Moro) |
| Year | 2020 |
| Volume | 77 |
| Issue | 3 |
| Pages | 235-242 |
| Publication date | 2020-08-12 |
| Peer Reviewed | Yes |
| Open Access | No |
| Type | ARTICLE |
| Venue | Anthropologischer Anzeiger (JOURNAL) |
| Journal identifiers | ISSN: 0003-5548 • E-ISSN: 2363-7099 |
| Publisher | S ch we iz er ba rt (PUBLISHER) |
| DOI | 10.1127/anthranz/2020/1162 |
| PMID | 32211746 |
| OpenAlex | W3013306015 |
| Language | EN |
DNA Trace Kit (Macherey NagelTM) protocol and an STR analysis was performed. Thanks to this modified protocol a complete DNA profile was obtained from the sternum bone, while only partial results were obtained from blood and teeth. The DNA profile obtained from the sternum was compared with the DNA of the putative son searching for a genetic match. Five incompatibilities were detected so it was possible to exclude the kinship. In conclusion this could be a useful technique in personal identification through DNA analysis in case of poor quality and quantity of bone.
Biology · DNA · DNA extraction · Gene · Polymerase chain reaction · Saponification · Sternum · Anatomy · Biochemistry · Forensic and Genetic Research · Genetics
| Citation velocity | historical |
|---|---|
| Highly cited | No |