Phase II validation study of the rK39 Elisa prototype for the diagnosis of canine visceral leishmaniasis in Brazil
Bibliographic Data
| ID | 5169841 |
|---|---|
| Authors | Fernanda Alvarenga Cardoso Medeiros (0000-0003-4315-3114, Ezequiel Dias Foundation), Job Alves De Souza Filho (0000-0002-6592-2378, Ezequiel Dias Foundation), José Ronaldo Barbosa (0000-0003-1838-368X, Ezequiel Dias Foundation), Lucas Edel Donato (0000-0001-5600-7891, Ministério da Saúde, Brazil), Fabiano Borges Figueiredo (0000-0001-6861-0997, Fundação Oswaldo Cruz), Guilherme Loureiro Werneck (0000-0003-1169-1436, Universidade do Estado do Rio de Janeiro), Gustavo Fontes Paz (0000-0002-5365-3393, Fundação Oswaldo Cruz), Mayerson Thompson (0000-0002-1207-3042, Universidade Federal de Minas Gerais), Andreza Pain Marcelino (0000-0002-7197-2128, Fundação Oswaldo Cruz) |
| Year | 2021 |
| Volume | 37 |
| Issue | 3 |
| Pages | e00041320-e00041320 |
| Publication date | 2021-01-01 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Cadernos de Saude Publica (JOURNAL) |
| Journal identifiers | ISSN: 0102-311X • E-ISSN: 1678-4464 |
| Publisher | FapUNIFESP (SciELO) (PUBLISHER) |
| DOI | 10.1590/0102-311x00041320 |
| PMID | 33852659 |
| OpenAlex | W3140732944 |
| SCIELO_PID | S0102-311X2021000305005 |
| Language | EN |
| References cited | 37 |
Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil
Canine leishmaniasis · Leishmania infantum · Leishmaniasis · Visceral leishmaniasis · Immunology · Leptospirosis research and findings · Medicine · Parasites and Host Interactions · Research on Leishmaniasis Studies · Virology
| Citation velocity | historical |
|---|---|
| Highly cited | No |