PCR in the investigation of canine American tegumentary leishmaniasis in northwestern Paraná State, Brazil
Bibliographic Data
American tegumentary leishmaniasis (ATL) was studied in 143 dogs in a rural area in the county of Mariluz, northwestern Paraná State, Brazil, using direct parasite search, indirect immunofluorescence (IIF), and polymerase chain reaction (PCR). Thirty-nine dogs (27.3%) presented lesions suggestive of the disease, 5 (12.8%) of which were positive in direct parasite search and PCR (lesion), and of these 5, 4 were also positive by IIF. Of the 34 dogs with negative direct parasite search, 12 (35.3%) had PCR- positive lesions, and of these, 5 were also IIF-positive. One hundred and four dogs had no lesions, but 17/101 (16.8%) were IIF-positive. PCR in blood was positive in 10/38 (26.3%) of the dogs with lesions and in 16/104 (15.4%) of dogs without lesions. The association between PCR (lesion or blood), direct parasite search, and IIF detected 24/39 (61.5%) positive results among symptomatic dogs and 31/104 (29.8%) among asymptomatic animals. PCR was useful for diagnosing ATL, but there was no correlation between lesions, serology, and plasma PCR. Furthermore, detection of parasite DNA in the blood may indicate hematogenous parasite dissemination
Geography · Leishmaniasis · Pathology · State (computer science) · Veterinary medicine · Medicine · Research on Leishmaniasis Studies
Pólos de produção de leishmaniose tegumentar americana no norte do Estado do Paraná, Brasil
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A serological and molecular investigation of American cutaneous leishmaniasis in dogs, three years after an outbreak in the Northwest of Paraná State, Brazil
| Unique citing works | 3 |
|---|---|
| Citations per year | 0,17 |
| Citation span | 2008 - 2009 (2) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 3 |