Mutations in the pfmdr1, cg2, and pfcrt genes in Plasmodium falciparum samples from endemic malaria areas in Rondonia and Pará State, Brazilian Amazon Region
Bibliographic Data
| ID | 5223954 |
|---|---|
| Authors | Giselle Maria Rachid Viana (0000-0003-1771-7211, Instituto Evandro Chagas), Ricardo Luiz Dantas Machado (0000-0002-8955-3204, Faculdade de Medicina de São José do Rio Preto), Ricardo Luís Dantas Machado (Faculdade de Medicina de São José do Rio Preto), Vanja Sueli Pachiano Calvosa (Instituto Evandro Chagas), Marinete Marins Póvoa (0000-0003-3517-2227, Instituto Evandro Chagas, corresponding author) |
| Year | 2006 |
| Volume | 22 |
| Issue | 12 |
| Pages | 2703-2711 |
| Publication date | 2006-12-01 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Cadernos de Saude Publica (JOURNAL) |
| Journal identifiers | ISSN: 0102-311X • E-ISSN: 1678-4464 |
| Publisher | FapUNIFESP (SciELO) (PUBLISHER) |
| DOI | 10.1590/s0102-311x2006001200019 |
| PMID | 17096048 |
| OpenAlex | W2159399552 |
| SCIELO_PID | S0102-311X2006001200019 |
| Language | EN |
| Citations received | 1 |
| References cited | 34 |
The objectives of this study were to investigate the molecular basis for Plasmodium falciparum resistance to chloroquine in isolates from the Brazilian Amazon and to identify polymorphisms in the pfmdr1 gene, codons 184, 1042, and 1246, the kappa and gamma regions of the cg2 gene, and the K76T mutation of the pfcrt gene, in order to calculate the distribution of polymorphism within each target gene, comparing samples from distinct geographic areas, using allele-specific polymerase chain reaction (PCR) for the pfmdr gene and PCR plus restriction fragment length polymorphism (RFLP) for the cg2 and pfcrt genes. The sample consisted of 40 human blood isolates, already collected and morphologically diagnosed as carriers of P. falciparum parasites, from four localities: Porto Velho in Rondonia State and Maraba, Itaituba, and Tailandia in Pará State. Distribution of P. falciparum in vitro chloroquine resistance in the isolates was 100% for pfmdr1, cg2 gamma region, and pfcrt, except for the polymorphism in the cg2 kappa region, which was not found
Allele · Apicomplexa · Biology · Gene · Malaria · Plasmodium falciparum · Polymerase chain reaction · Restriction fragment length polymorphism · Genetics · Immunology · Malaria Research and Control · Mosquito-borne diseases and control · Parasites and Host Interactions · Virology
| Unique citing works | 1 |
|---|---|
| Citations per year | 0,07 |
| Citation span | 2012 - 2012 (1) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 1 |