Classic Liver Lobule as a Model for Studying the Influence of Cadmium Subtoxic Doses

The goal was to study the liver structural features of Wistar -rat-male-adolescents under sudacute influence of cadmium sulphate. The study was carried out by means of optical microscopy (Axio Scope.Al, С. Zeiss) and submicroscopy (JEM 100-S и JEM 1400) taking into account the classic liver lobule which is the liver tissular model with the arterial-venous gradient coinciding with a cell turnover vector. To assess the significancy of tissular and ultrastructural stereological differences between the groups the nonparametric Mann-Whitney method was used. The lobular topographical features of alterative and regenerative reactions of adolescents were revealed on tissular and cell levels. Hepatocyte destruction is associated with single lymphocytes and macrophages, moving from the expanded perivenular sinusoids. The tissular stereological analysis indicated the synchronous growth of space density of parenchymatous liver compartment and liver sinusoids of rats with toxic influence compared to the control group. According to the stereological analysis of hepatocytes ultrastructural organization a progressive space density decrease of the biosynthetic organelles - of granular cytoplasmic reticulum and mitochondria was revealed. We observed reduction of cytoplasmic reticulum profiles with preservation of its perinuclear compartments and ultrastructural equivalents of "stress" - the folds of osmiophylic membranes in giant vacuoles. A certain balance between alterative processes of toxic genesis and compensatory processes in the liver parenchyma of male-adolescents Wistar was revealed

Biology · Cytoplasm · Endoplasmic reticulum · Hepatocyte · Lobules of liver · Parenchyma · Pathology · Sinusoid · Stereology · Ultrastructure · Chemistry · Immunotoxicology and immune responses · Medicine · Anatomy · Biochemistry · Cell Biology · Endocrinology