Characterization of the D18S535 STR Locus in Northern Ontario European and Aboriginal Populations for Forensic Purposes
Bibliographic Data
| ID | 7990317 |
|---|---|
| Authors | Krista A Currie (Laurentian University), Paul A M Michael, P Michael, K K Nkongolo (0000-0001-9269-318X) |
| Year | 2005 |
| Volume | 77 |
| Issue | 2 |
| Pages | 267-279 |
| Publication date | 2005-01-01 |
| Peer Reviewed | Yes |
| Open Access | No |
| Type | ARTICLE |
| Venue | Human Biology (JOURNAL) |
| Journal identifiers | ISSN: 0018-7143 • E-ISSN: 1534-6617 |
| Publisher | Project MUSE (PUBLISHER • US) |
| DOI | 10.1353/hub.2005.0038 |
| PMID | 16201142 |
| OpenAlex | W2090327464 |
| Language | EN |
| Citations received | 1 |
| References cited | 13 |
Genetic characterization of one European and three aboriginal populations from northern Ontario was undertaken to assess the utility of the D18S535 short tandem repeat locus (STR) as a genetic marker for forensic DNA typing in the region. The D18S535 locus was amplified using monoplex polymerase chain reaction (PCR), separated by denaturing polyacrylamide gel electrophoresis (PAGE), and visualized using the silver-stain detection method. The generated population data demonstrated that the D18S535 locus is highly polymorphic with a heterozygosity of > or = 0.75. The exact test showed violations of Hardy-Weinberg equilibrium in two of the aboriginal populations. Pairwise comparisons of allele-frequency distributions indicated that the four northern Ontario populations were significantly different from each other. This test also revealed that the northern Ontario populations differed significantly from ten European populations (from Germany, Spain, and Croatia) and one population from South America (from Argentina). Forensic parameters showed that the D18S535 locus is highly discriminating (power of discrimination > or = 0.85, chance of exclusion > or = 0.51); however, the lack of Hardy-Weinberg equilibrium in some of the populations must be taken into account in the application of these results to northern Ontario forensic casework
Allele · Allele frequency · Biology · DNA · DNA profiling · Evolutionary biology · Forensic identification · Forensic science · Gene · Geography · Locus (genetics · Loss of heterozygosity · Microsatellite · Polymerase chain reaction · Population · Population genetics · Demography · Environmental DNA in Biodiversity Studies · Forensic and Genetic Research · Genetics · Molecular Biology Techniques and Applications
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| Unique citing works | 1 |
|---|---|
| Citations per year | 0,07 |
| Citation span | 2012 - 2012 (1) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 1 |