Brief communication

Minimally invasive bone sampling method for DNA analysis

Dados Bibliográficos

ID8317056
AutoresV E Gibbons (0000-0001-7875-3297, University of the Witwatersrand, autor correspondente), Victoria E Gibbon, Clem B Penny, Clem Penny (University of the Witwatersrand), Goran Strkalj (0000-0003-0973-0707, Macquarie University), Paul Ruff (0000-0003-1055-1730, University of the Witwatersrand)
Ano2009
Volume139
Fascículo4
Páginas596-599
Data de publicação2009-08-01
Peer ReviewedSim
Open AccessSim
TipoARTICLE
PeriódicoAmerican Journal of Physical Anthropology (JOURNAL)
Identificadores do periódicoISSN: 0002-9483 • E-ISSN: 1096-8644
EditoraWiley (PUBLISHER • GB)
DOI10.1002/ajpa.21048
PMID19350643
OpenAlexW2072507577
IdiomaEN
Citações recebidas1
Referências citadas22

Obtaining a bone sample for DNA analysis has traditionally been a destructive practice, which has resulted in reluctance on behalf of curators for skeletal collections to allow invasive testing. A novel minimally invasive bone sampling method for DNA analysis is presented here. This method uses a conventional hand drill wherein the bone sample is extracted from the intercondylar fossa of the femur; it does not interfere with any known anthropometric landmarks and only leaves a small hole on the surface of the bone. The temperature of the drill is documented and it was established due to the minor increase in temperature, that this should not affect the molecular integrity of the sample. This method is easily replicated and is suitable for both human and other animal skeletal material and can be applied to rare specimens with little risk. Am J Phys Anthropol, 2009. © 2009 Wiley‐Liss, Inc

Chromatography · Computer vision · Drill · Femur · Sample (material) · Sampling (signal processing) · Biomedical Engineering · Chemistry · Computer Science · Dentistry · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Materials Science · Medicine · Molecular Biology Techniques and Applications · Surgery