Reproducibility of aDNA typing
Bibliographic Data
| ID | 3778298 |
|---|---|
| Authors | Wera M Schmerer (0000-0003-2467-1362, University of Göttingen), Hummel (0000-0002-3647-8968), Susanne Hummel, Bernd Herrmann (0009-0000-1851-8746) |
| Year | 1997 |
| Volume | 55 |
| Issue | 2 |
| Pages | 199-206 |
| Publication date | 1997-06-01 |
| Peer Reviewed | Yes |
| Open Access | No |
| Type | ARTICLE |
| Venue | Anthropologischer Anzeiger (JOURNAL) |
| Journal identifiers | ISSN: 0003-5548 • E-ISSN: 2363-7099 |
| Publisher | S ch we iz er ba rt (PUBLISHER) |
| DOI | 10.1127/anthranz/55/1997/199 |
| PMID | 9341087 |
| OpenAlex | W2405614169 |
| Language | EN |
| Citations received | 2 |
The reproducibility of short tandem repeat (STR) amplification of aDNA extracts is limited by means of formation of artifacts during PCR. One of these artifacts, the so called allelic-dropout (failure of the amplification of alleles), may result in false-homozygote typing of a sample, if genotyping is based on the analysis of a single amplification product. 30 tooth- and bone samples collected from 17 individuals of three burial sites were investigated in a blind test. Using the polymerase chain reaction (PCR) the two independent segregating STR-loci HUMVWA and HUMTH01 were amplified. Corresponding to a mathematical estimation, multiple amplifications of each sample and genelocus were carried out. The results of this genotyping were compared intraindividually.
Allele · Ancient DNA · Biology · Chromatography · Gene · Genotype · Genotyping · Microsatellite · Polymerase chain reaction · Population · Reproducibility · Typing · Chemistry · Dental Trauma and Treatments · Forensic Anthropology and Bioarchaeology Studies · Genetics · Medicine · Molecular Biology Techniques and Applications
| Unique citing works | 2 |
|---|---|
| Citations per year | 0,08 |
| Citation span | 2000 - 2004 (5) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 2 |