Chengtao Li
Biographic Data
| ID | 4298662 |
|---|---|
| NAME | Chengtao Li |
| GIVEN NAMES | Chengtao |
| FAMILY NAME | Li |
| SIGNATURE | LI C |
| AFFILIATIONS | Institute of Forensic Science |
| ORCID | 0000-0001-6852-9144 |
| VERIFIED | Yes |
| TOTAL WORKS | 19 |
| TOTAL CITATIONS | 6 |
| AUTHOR COUNT | 19 |
| EDITOR COUNT | 0 |
| FIRST PUBLICATION YEAR | 2017 |
| LATEST PUBLICATION YEAR | 2025 |
| H-INDEX | 1 |
Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform
Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of fo…
Quantitation of human mitochondrial DNA and whole mtGenomes sequencing of fingernail/hair shaft samples
The analysis of mitochondrial DNA (mtDNA) is suitable for fingernail/hair shaft samples in forensic science. A successful mtDNA forensic analysis depends on the quantity and quality of the mtDNA. The application of massively parallel sequence techniques to the analysis of mtDNA has the potential to improve the recovery of genetic information from difficult forensic specimens and to increase the discrimination potential of mtDNA by capturing and c…
Validation and forensic application of a new 36 X-chromosomal short tandem repeat loci multiplex system
X-chromosomal short tandem repeats are indispensable in specific cases, distinct from autosomal and Y chromosome genetic markers. SureID(R) X37 is an innovative six-colour fluorescence multiplex detection system that can simultaneously amplify 36 X-chromosomal short tandem repeat loci (including DXS6795, DXS7132, DXS8378, DXS10101, DXS10103, DXS10079, DXS10134, GATA165B12, GATA172D05, HPRTB, DXS6810, DXS10135, DXS6797, DXS10074, DXS7424, DXS9902,…
Forensic features and phylogenetic structure survey of four populations from southwest China via the autosomal insertion/deletion markers
InDel markers show promise for forensic individual identification and parentage testing via the AGCU InDel 50 kit.Genetic analysis of Guizhou populations reveals correlations with linguistic, geographical, and cultural factors.Guizhou Han, Dong, and Chuanqing populations showed closer genetic affiliations with linguistically similar groups than the Guizhou Yi
Internal validation of an improved system for forensic application
A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application.The multiplex included rapidly mutating Y-STRs and low-medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits.The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases
Development of forensic standards in China
Forensic science is crucial for the administration of justice and case investigation. In China, political-legal organizations, including the courts, public security, procuratorate, and judicial administration, developed their own forensic practices before 2004. As a result, the frequent and repeated appraisals undermined judicial authority and credibility. Thus, a law was published in 2005 to improve the uniform forensic management system by the …
Investigation on the Genetic-Inconsistent Paternity Cases Using the MiSeq FGx System
Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity ca…
Regulatory Variation within 3’UTR of STAT5A Correlates with Sudden Cardiac Death in Chinese Populations
Definitive diagnosis to sudden cardiac death (SCD) is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death. It is therefore important to uncover the inherited risk component to SCD. Signal transducer and activators of transcription 5 A (STAT5A) is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular …
Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application
Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East A…
Multi-Locus Identification of Psilocybe Cubensis by High-Resolution Melting (HRM)
Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Genetic investigation and phylogenetic analysis of three Chinese ethnic groups using 16 X chromosome STR loci
Background: The value of using X-chromosomal short tandem repeats (X-STRs) as genetic markers in human genetics has been widely recognised. However, the 16 X-STRs in the Goldeneye ® DNA ID System 17X kit have not been thoroughly applied. Aim: To investigate the genetic polymorphisms of 16 X-STRs in three main ethnic minorities (Tibetan, Mongolian and Kazakh) in China and to reveal the phylogenetic relationships of different populations. Subjects …
Genetic polymorphisms of 21 STR loci of Golden e yeTM DNA ID 22NC kit in five ethnic groups of China
Dear Editor, Short tandem repeats (STRs), polymorphic DNA regions with a variable number of repeated units (2–6 base pairs), are attractive to forensic applications such as human identification and
Genetic characterization of four dog breeds with Illumina CanineHD BeadChip
Dear Editor, Due to human's cohabitation with domesticated animals, molecular analysis of animal DNA is increasingly being admitted as evidence in forensic investigations. In 2011, recommendations
Validation studies of the ParaDNA ® Intelligence System with artificial evidence items
Short tandem repeat (STR) profiling is one of the mostly used systems for forensic applications. In certain circumstances, STR profiling is time-consuming and costly, which potentially leads to delays in criminal investigations. LGC (Laboratory of the Government Chemist, UK) Forensics has developed a robust STR profiling platform called the ParaDNA ® Intelligence Test System which can provide early tactical intelligence and aid investigators in m…
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Forensic genetics
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Massively parallel sequencing of 231 autosomal SNPs with a custom panel
The custom-designed single nucleotide polymorphism (SNP) panel amplified 231 autosomal SNPs in one PCR reaction and subsequently sequenced with massively parallel sequencing (MPS) technology and Ion Torrent personal genome machine (PGM). SNPs were chosen from SNPforID, IISNP, HapMap, dbSNP, and related published literatures. Full concordance was obtained between available MPS calling and Sanger sequencing with 9947A and 9948 controls. Ten SNPs (r…
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Massively parallel sequencing of 231 autosomal SNPs with a custom panel
The custom-designed single nucleotide polymorphism (SNP) panel amplified 231 autosomal SNPs in one PCR reaction and subsequently sequenced with massively parallel sequencing (MPS) technology and Ion Torrent personal genome machine (PGM). SNPs were chosen from SNPforID, IISNP, HapMap, dbSNP, and related published literatures. Full concordance was obtained between available MPS calling and Sanger sequencing with 9947A and 9948 controls. Ten SNPs (r…
Forensic genetics
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Genetic polymorphisms of 21 STR loci of Golden e yeTM DNA ID 22NC kit in five ethnic groups of China
Dear Editor, Short tandem repeats (STRs), polymorphic DNA regions with a variable number of repeated units (2–6 base pairs), are attractive to forensic applications such as human identification and
Genetic characterization of four dog breeds with Illumina CanineHD BeadChip
Dear Editor, Due to human's cohabitation with domesticated animals, molecular analysis of animal DNA is increasingly being admitted as evidence in forensic investigations. In 2011, recommendations
Validation studies of the ParaDNA ® Intelligence System with artificial evidence items
Short tandem repeat (STR) profiling is one of the mostly used systems for forensic applications. In certain circumstances, STR profiling is time-consuming and costly, which potentially leads to delays in criminal investigations. LGC (Laboratory of the Government Chemist, UK) Forensics has developed a robust STR profiling platform called the ParaDNA ® Intelligence Test System which can provide early tactical intelligence and aid investigators in m…
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Genetic investigation and phylogenetic analysis of three Chinese ethnic groups using 16 X chromosome STR loci
Background: The value of using X-chromosomal short tandem repeats (X-STRs) as genetic markers in human genetics has been widely recognised. However, the 16 X-STRs in the Goldeneye ® DNA ID System 17X kit have not been thoroughly applied. Aim: To investigate the genetic polymorphisms of 16 X-STRs in three main ethnic minorities (Tibetan, Mongolian and Kazakh) in China and to reveal the phylogenetic relationships of different populations. Subjects …
Regulatory Variation within 3’UTR of STAT5A Correlates with Sudden Cardiac Death in Chinese Populations
Definitive diagnosis to sudden cardiac death (SCD) is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death. It is therefore important to uncover the inherited risk component to SCD. Signal transducer and activators of transcription 5 A (STAT5A) is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular …
Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application
Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East A…
Multi-Locus Identification of Psilocybe Cubensis by High-Resolution Melting (HRM)
Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Development of forensic standards in China
Forensic science is crucial for the administration of justice and case investigation. In China, political-legal organizations, including the courts, public security, procuratorate, and judicial administration, developed their own forensic practices before 2004. As a result, the frequent and repeated appraisals undermined judicial authority and credibility. Thus, a law was published in 2005 to improve the uniform forensic management system by the …
Investigation on the Genetic-Inconsistent Paternity Cases Using the MiSeq FGx System
Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity ca…
Internal validation of an improved system for forensic application
A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application.The multiplex included rapidly mutating Y-STRs and low-medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits.The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases
Forensic features and phylogenetic structure survey of four populations from southwest China via the autosomal insertion/deletion markers
InDel markers show promise for forensic individual identification and parentage testing via the AGCU InDel 50 kit.Genetic analysis of Guizhou populations reveals correlations with linguistic, geographical, and cultural factors.Guizhou Han, Dong, and Chuanqing populations showed closer genetic affiliations with linguistically similar groups than the Guizhou Yi
Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform
Massive parallel sequencing (MPS) has rapidly emerged as a promising technique for forensic DNA typing due to its capacity to simultaneously detect numerous genetic markers and samples in a single reaction, allowing the direct acquisition of sequence information. In this current investigation, the FGID forensic four-in-one DNA typing kit was employed on the DNBSEQ-G99RS high-throughput sequencing platform to simultaneously analyse two types of fo…
Quantitation of human mitochondrial DNA and whole mtGenomes sequencing of fingernail/hair shaft samples
The analysis of mitochondrial DNA (mtDNA) is suitable for fingernail/hair shaft samples in forensic science. A successful mtDNA forensic analysis depends on the quantity and quality of the mtDNA. The application of massively parallel sequence techniques to the analysis of mtDNA has the potential to improve the recovery of genetic information from difficult forensic specimens and to increase the discrimination potential of mtDNA by capturing and c…
Validation and forensic application of a new 36 X-chromosomal short tandem repeat loci multiplex system
X-chromosomal short tandem repeats are indispensable in specific cases, distinct from autosomal and Y chromosome genetic markers. SureID(R) X37 is an innovative six-colour fluorescence multiplex detection system that can simultaneously amplify 36 X-chromosomal short tandem repeat loci (including DXS6795, DXS7132, DXS8378, DXS10101, DXS10103, DXS10079, DXS10134, GATA165B12, GATA172D05, HPRTB, DXS6810, DXS10135, DXS6797, DXS10074, DXS7424, DXS9902,…
Biology (17 works) · Forensic and Genetic Research (14 works) · Gene (14 works) · Genetics (12 works) · Molecular Biology Techniques and Applications (11 works) · Genotype (10 works) · DNA (8 works) · Microsatellite (8 works) · Allele (7 works) · Single-nucleotide polymorphism (7 works)