B L Lasley
Biographic Data
| ID | 5433220 |
|---|---|
| NAME | B L Lasley |
| GIVEN NAMES | B L |
| FAMILY NAME | Lasley |
| SIGNATURE | LASLEY B L |
| AFFILIATIONS | University of California, Davis |
| VERIFIED | No |
| TOTAL WORKS | 16 |
| TOTAL CITATIONS | 0 |
| WORKS AS AUTHOR | 16 |
| WORKS AS EDITOR | 0 |
| FIRST PUBLICATION YEAR | 1982 |
| LATEST PUBLICATION YEAR | 2003 |
| H-INDEX | 0 |
Contribution of ovarian steroid production to urinary estrone conjugate concentrations in Macaca mulatta
This study was designed to test the hypothesis that basal estrone conjugate (E 1 C) profiles do not accurately detect ovarian function when ovarian estrogen production is low or absent. We employed surgical removal of active ovaries from laboratory rhesus macaques to simulate an acute decline in ovarian estrogen production. In the first experiment, urine samples collected prior to and following ovariectomy (Ovx) were subjected to high‐performance…
Excretion and measurement of estradiol and progesterone metabolites in the feces and urine of female squirrel monkeys ( Saimiri sciureus )
The first objective of the present study was to determine the metabolic form and rate of excretion of ovarian hormone metabolites in the urine and feces of female squirrel monkeys injected with radiolabeled progesterone (Po) and estradiol. The major portion of the urinary metabolites of both hormones was excreted within 16–24 hr post‐injection. Estrogen and Po isotopes in feces exhibited an excretion peak at 16 hr post‐injection. The majority of …
Fecal analysis of ovarian cycles in female black‐handed spider monkeys ( Ateles geoffroyi )
An enzyme immunoassay (EIA) was applied to characterize the reproductive endocrinology of adult female black‐handed spider monkeys ( Ateles geoffroyi ). Analysis of paired urine and fecal samples, collected from two females housed at San Diego Zoo, confirmed that the EIAs employed provided quantitative measurements of ovarian sex steroid hormones. Fecal metabolite levels were significantly correlated with those in urine, confirming that feces are…
Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques
An enzyme-linked immunosorbent assay (ELISA) for human urinary beta follicle-stimulating hormone (FSH) subunit was validated for use in the laboratory macaque (Macaca mulatta and Macaca fasicularis). This ELISA is based on the dissociation of the FSH heterodimer in urine and the subsequent measurement of the beta subunit as a representation of total urinary FSH. This assay was then used to describe the gonadotropin escape following ovarian senesc…
Reproductive biology of female titi monkeys (Callicebus moloch) in captivity
Titi monkeys (Callicebus spp., Cebidae) are monogamous neotropical primates that live in family-like groups typically consisting of an adult monogamous pair and one or two young. Knowledge about the reproductive biology of this genus is scanty. This study investigated the reproductive biology of female dusky titi monkeys (Callicebus moloch). An initial analysis characterized reproductive parameters of 32 females from a captive colony maintained f…
Elisa for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the beta subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has be…
Urine and fecal sample collection on filter paper for ovarian hormone evaluations
A practical method for collecting, storing, and transporting liquid biological samples in a dry state for subsequent hormone metabolite analyses is presented. This method employs the use of ordinary filter paper strips that imbibe liquid samples. Samples taken up by the filter paper were allowed to dry and were retained at ambient conditions in capped vials for up to 5 years prior to analysis. Examples presented in the present report include urin…
Monitoring female reproductive function by measurement of fecal estrogen and progesterone metabolites in the white‐faced saki ( Pithecia pithecia )
A simple method for extracting and measuring ovarian steroids in feces is applied to the ovarian cycle, pregnancy, parturition, and period of lactational amenorrhea in Pithecia pithecia . Small amounts of wet, unmixed feces were combined with a modified phosphate buffer, shaken, centrifuged, and decanted, and the supernatant was directly measured for estrogen and progesterone metabolites by enzyme immunoassays. Urinary estrogen and progesterone m…
Assessment of luteal competency by urinary hormone evaluation in the captive female gorilla
Analysis of urinary estrone conjugates and pregnanediol‐3‐glucuronide were used to document ovarian events in female gorillas known to be fertile. These normal parameters were used to assess ovarian function in gorillas suspected as infertile. Insufficient luteal hormonal dynamics were observed in the infertile group. Based on this observation, exogenous progesterone as the only treatment was administered to infertile females post ovulation and m…
The relationship of serum estradiol and progesterone concentrations to the enzyme immunoassay measurements of urinary estrone conjugates and immunoreactive pregnanediol‐3‐glucuronide in Macaca mulatta
Paired urine and serum samples from four conceptive and six nonconceptive ovarian cycles of seven adult Macaca mullatta were analyzed by radioimmunoassay (RIA) for circulating estradiol (E 2 ) and progesterone (Po), and urinary estrone conjugates (E 1 C) and immunoreactive preganediol‐3‐glucuronide (iPDG) using enzyme immunoassay (EIA). Nonconceptive cycles exhibited a fivefold increase in urinary E 1 C and serum E 2 levels from follicular phase …
Excretion of estrone, estradiol, and progesterone in the urine and feces of the female cotton‐top tamarin ( Saguinus oedipus oedipus )
The excretion of three gonadal steroids was studied in the urine and feces of female cotton‐top tamarins ( Saguinus oedipus oedipus ). Each steroid, 14 C‐estrone, 14 C‐estradiol, and 14 C‐progesterone, was injected into a separate female cotton‐top tamarin. Urine and feces were collected at 8 hr intervals for 5 days on the three tamarins. Samples were analyzed to determine the proportion of free and conjugated steroids. Steroid excretion patterns…
Direct measurements of urinary estrone conjugates during the normal menstrual cycle of the gorilla ( Gorilla gorilla )
A direct immunoassay for urinary estrone conjugates (estrone sulfate and estrone glucuronide) was used to assess the preovulatory estrogen rise in normal gorilla menstrual cycles. Immunoreactive estrone conjugates in samples concomitantly assessed for total estrogen immunoreactivity reflected similar profiles throughout the cycle; however, the speed and resolution of the direct assay for conjugates indicate this method to be more accurate in moni…
Sexual behavior and urinary ovarian hormone concentrations during the lowland Gorilla menstrual cycle
A practical approach to evaluation of fertility in the female gorilla
Measurements of urinary estrogen, pregnanediol-3-glucuronide, and bioactive luteinizing hormone indexed by creatinine on a daily basis in female gorillas reflect ovarian function. The use of small urine samples obviates the need for capture, restraint, or isolation and provides the basis for a nonstressful evaluation of reproductive status or fertility. Initial attempts at artificial insemination have thus far been unsuccessful when the fertile p…
A comparison of primate ovarian cycles
In an attempt to relate sexual behavior to gonadal steroid production in an array of primate species, urinary estrogen and behavior were correlated in both estrous and menstrual-cycle-type species. Small daily urine samples were collected each morning from ruffed lemur, lion-tailed macaque, and gorilla. These were evaluated for total immunoreactive estrogen to monitor ovarian follicular growth and the time of ovulation in all species. Broadly com…
Urinary immunoreactive estrogen and pregnanediol‐3‐glucuronide during the normal menstrual cycle of the female lowland gorilla ( Gorilla gorilla )
Urine samples were taken daily during 22 menstrual cycles of six normal adult female gorillas. Urine was analyzed for total immunoreactive estrogens (E t ) and pregnanediol‐3‐glucuronide (PdG) and indexed by creatinine (Cr). An average cycle length of 32 ± 1 days (mean ± SE) with a range of 25–42 days is reported. Estrogen values range from 4 to 128 ng/mg Cr and show a midcycle peak and a midluteal rise. PdG values range from 0.01 to 2.4 μg/mg Cr…
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A practical approach to evaluation of fertility in the female gorilla
Measurements of urinary estrogen, pregnanediol-3-glucuronide, and bioactive luteinizing hormone indexed by creatinine on a daily basis in female gorillas reflect ovarian function. The use of small urine samples obviates the need for capture, restraint, or isolation and provides the basis for a nonstressful evaluation of reproductive status or fertility. Initial attempts at artificial insemination have thus far been unsuccessful when the fertile p…
A comparison of primate ovarian cycles
In an attempt to relate sexual behavior to gonadal steroid production in an array of primate species, urinary estrogen and behavior were correlated in both estrous and menstrual-cycle-type species. Small daily urine samples were collected each morning from ruffed lemur, lion-tailed macaque, and gorilla. These were evaluated for total immunoreactive estrogen to monitor ovarian follicular growth and the time of ovulation in all species. Broadly com…
Urinary immunoreactive estrogen and pregnanediol‐3‐glucuronide during the normal menstrual cycle of the female lowland gorilla ( Gorilla gorilla )
Urine samples were taken daily during 22 menstrual cycles of six normal adult female gorillas. Urine was analyzed for total immunoreactive estrogens (E t ) and pregnanediol‐3‐glucuronide (PdG) and indexed by creatinine (Cr). An average cycle length of 32 ± 1 days (mean ± SE) with a range of 25–42 days is reported. Estrogen values range from 4 to 128 ng/mg Cr and show a midcycle peak and a midluteal rise. PdG values range from 0.01 to 2.4 μg/mg Cr…
Sexual behavior and urinary ovarian hormone concentrations during the lowland Gorilla menstrual cycle
Direct measurements of urinary estrone conjugates during the normal menstrual cycle of the gorilla ( Gorilla gorilla )
A direct immunoassay for urinary estrone conjugates (estrone sulfate and estrone glucuronide) was used to assess the preovulatory estrogen rise in normal gorilla menstrual cycles. Immunoreactive estrone conjugates in samples concomitantly assessed for total estrogen immunoreactivity reflected similar profiles throughout the cycle; however, the speed and resolution of the direct assay for conjugates indicate this method to be more accurate in moni…
Excretion of estrone, estradiol, and progesterone in the urine and feces of the female cotton‐top tamarin ( Saguinus oedipus oedipus )
The excretion of three gonadal steroids was studied in the urine and feces of female cotton‐top tamarins ( Saguinus oedipus oedipus ). Each steroid, 14 C‐estrone, 14 C‐estradiol, and 14 C‐progesterone, was injected into a separate female cotton‐top tamarin. Urine and feces were collected at 8 hr intervals for 5 days on the three tamarins. Samples were analyzed to determine the proportion of free and conjugated steroids. Steroid excretion patterns…
The relationship of serum estradiol and progesterone concentrations to the enzyme immunoassay measurements of urinary estrone conjugates and immunoreactive pregnanediol‐3‐glucuronide in Macaca mulatta
Paired urine and serum samples from four conceptive and six nonconceptive ovarian cycles of seven adult Macaca mullatta were analyzed by radioimmunoassay (RIA) for circulating estradiol (E 2 ) and progesterone (Po), and urinary estrone conjugates (E 1 C) and immunoreactive preganediol‐3‐glucuronide (iPDG) using enzyme immunoassay (EIA). Nonconceptive cycles exhibited a fivefold increase in urinary E 1 C and serum E 2 levels from follicular phase …
Assessment of luteal competency by urinary hormone evaluation in the captive female gorilla
Analysis of urinary estrone conjugates and pregnanediol‐3‐glucuronide were used to document ovarian events in female gorillas known to be fertile. These normal parameters were used to assess ovarian function in gorillas suspected as infertile. Insufficient luteal hormonal dynamics were observed in the infertile group. Based on this observation, exogenous progesterone as the only treatment was administered to infertile females post ovulation and m…
Monitoring female reproductive function by measurement of fecal estrogen and progesterone metabolites in the white‐faced saki ( Pithecia pithecia )
A simple method for extracting and measuring ovarian steroids in feces is applied to the ovarian cycle, pregnancy, parturition, and period of lactational amenorrhea in Pithecia pithecia . Small amounts of wet, unmixed feces were combined with a modified phosphate buffer, shaken, centrifuged, and decanted, and the supernatant was directly measured for estrogen and progesterone metabolites by enzyme immunoassays. Urinary estrogen and progesterone m…
Urine and fecal sample collection on filter paper for ovarian hormone evaluations
A practical method for collecting, storing, and transporting liquid biological samples in a dry state for subsequent hormone metabolite analyses is presented. This method employs the use of ordinary filter paper strips that imbibe liquid samples. Samples taken up by the filter paper were allowed to dry and were retained at ambient conditions in capped vials for up to 5 years prior to analysis. Examples presented in the present report include urin…
Elisa for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the beta subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has be…
Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques
An enzyme-linked immunosorbent assay (ELISA) for human urinary beta follicle-stimulating hormone (FSH) subunit was validated for use in the laboratory macaque (Macaca mulatta and Macaca fasicularis). This ELISA is based on the dissociation of the FSH heterodimer in urine and the subsequent measurement of the beta subunit as a representation of total urinary FSH. This assay was then used to describe the gonadotropin escape following ovarian senesc…
Reproductive biology of female titi monkeys (Callicebus moloch) in captivity
Titi monkeys (Callicebus spp., Cebidae) are monogamous neotropical primates that live in family-like groups typically consisting of an adult monogamous pair and one or two young. Knowledge about the reproductive biology of this genus is scanty. This study investigated the reproductive biology of female dusky titi monkeys (Callicebus moloch). An initial analysis characterized reproductive parameters of 32 females from a captive colony maintained f…
Fecal analysis of ovarian cycles in female black‐handed spider monkeys ( Ateles geoffroyi )
An enzyme immunoassay (EIA) was applied to characterize the reproductive endocrinology of adult female black‐handed spider monkeys ( Ateles geoffroyi ). Analysis of paired urine and fecal samples, collected from two females housed at San Diego Zoo, confirmed that the EIAs employed provided quantitative measurements of ovarian sex steroid hormones. Fecal metabolite levels were significantly correlated with those in urine, confirming that feces are…
Excretion and measurement of estradiol and progesterone metabolites in the feces and urine of female squirrel monkeys ( Saimiri sciureus )
The first objective of the present study was to determine the metabolic form and rate of excretion of ovarian hormone metabolites in the urine and feces of female squirrel monkeys injected with radiolabeled progesterone (Po) and estradiol. The major portion of the urinary metabolites of both hormones was excreted within 16–24 hr post‐injection. Estrogen and Po isotopes in feces exhibited an excretion peak at 16 hr post‐injection. The majority of …
Contribution of ovarian steroid production to urinary estrone conjugate concentrations in Macaca mulatta
This study was designed to test the hypothesis that basal estrone conjugate (E 1 C) profiles do not accurately detect ovarian function when ovarian estrogen production is low or absent. We employed surgical removal of active ovaries from laboratory rhesus macaques to simulate an acute decline in ovarian estrogen production. In the first experiment, urine samples collected prior to and following ovariectomy (Ovx) were subjected to high‐performance…
Biology (16 works) · Medicine (15 works) · Hormone (13 works) · Internal Medicine (13 works) · Endocrinology (12 works) · Urine (12 works) · Estrogen (9 works) · Urinary system (9 works) · Chemistry (7 works) · Physiology (7 works)