Elisa for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
Bibliographic Data
| ID | 22898916 |
|---|---|
| Authors | C J Munro, Coralie Munro (University of California, Davis, corresponding author), Lisa S Laughlin (University of California, Davis), Juan Carlos Illera (0000-0002-9178-3121, University of California, Davis), Jacquelyn A Dieter (University of California, Davis), J Dieter, A G Hendrickx (University of California, Davis), B L Lasley (University of California, Davis) |
| Year | 1997 |
| Volume | 41 |
| Issue | 4 |
| Pages | 307-322 |
| Publication date | 1997-01-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | American Journal of Primatology (JOURNAL) |
| Journal identifiers | ISSN: 0275-2565 • E-ISSN: 1098-2345 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1002/(sici)1098-2345(1997)41:4<307::aid-ajp3>3.0.co;2-0 |
| PMID | 9093694 |
| OpenAlex | W2079527564 |
| Language | EN |
| Citations received | 3 |
| References cited | 20 |
A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the beta subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has been shown to detect LH and CG by radioimmunoassay (RIA) in both human and non-human primates. A polyclonal antiserum raised in rabbits against human chorionic gonadotropin (hCG) is conjugated to horseradish peroxidase, and is used as the second antibody signal. This anti-hCG antiserum cross reacts with CG secreted by both the human (hCG) and macaque (mCG). The ELISA utilizes hCG as the standard, and results are based on the relative concentrations of mCG in serum and urine. Total assay time is less than 5 hours. Range of the standard curve is 0.002 to 0.5 ng hCG/well, and the least detectable concentration of hCG is 0.0023 +/- 0.0007 ng/well. Pregnancy was detected in early pregnant macaques (M. fascicularis) on 9 (N = 1/16), 10 (N = 1/16), 11 (N = 1/16), 12 (N = 6/16), 13 (N = 1/16), 14 (N = 4/16), and 15 (N = 2/16) days following the pre-ovulatory urinary estrone conjugate peak. The detection of pregnancy by urinary mCG occurred approximately 24 to 72 hours after its detection in serum.
Antibody · Antiserum · Biology · Gonadotropin · Hormone · Human chorionic gonadotropin · Luteinizing hormone · Monoclonal antibody · Polyclonal antibodies · Radioimmunoassay · Urinary system · Urine · Animal Genetics and Reproduction · Chemistry · Endocrinology · Hypothalamic control of reproductive hormones · Immunology · Internal Medicine · Medicine · Reproductive Physiology in Livestock
Noninstrumented enzyme‐linked immunosorbant assay for detection of early pregnancy in macaques
Comparison of chorionic gonadotropin expression in human and macaque ( Macaca fascicularis ) trophoblasts
Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques
Detection of urinary gonadotropins in callitrichid monkeys with a sensitive immunoassay based upan a unique monoclonal antibody
Use of a radioreceptorassay (RRA) for human luteinizing hormone/chorionic gonadotropin (hLH/CG) for detection of early pregnancy and estimation of time of ovulation in macaques
Serum chorionic gonadotropin levels determined by radioreceptorassay and early diagnosis of pregnancy in the cynomolgus monkey ( Macaca fascicularis )
| Citations per year | 0.11 |
|---|---|
| Citation span | 1999 - 2002 (4) |
| Highly cited | No |