Lisa S Laughlin
Biographic Data
| ID | 8907190 |
|---|---|
| NAME | Lisa S Laughlin |
| GIVEN NAMES | Lisa S |
| FAMILY NAME | Laughlin |
| SIGNATURE | LAUGHLIN L S |
| AFFILIATIONS | University of California, Davis |
| VERIFIED | No |
| TOTAL WORKS | 6 |
| TOTAL CITATIONS | 0 |
| WORKS AS AUTHOR | 6 |
| WORKS AS EDITOR | 0 |
| FIRST PUBLICATION YEAR | 1990 |
| LATEST PUBLICATION YEAR | 2003 |
| H-INDEX | 0 |
Contribution of ovarian steroid production to urinary estrone conjugate concentrations in Macaca mulatta
This study was designed to test the hypothesis that basal estrone conjugate (E 1 C) profiles do not accurately detect ovarian function when ovarian estrogen production is low or absent. We employed surgical removal of active ovaries from laboratory rhesus macaques to simulate an acute decline in ovarian estrogen production. In the first experiment, urine samples collected prior to and following ovariectomy (Ovx) were subjected to high‐performance…
Comparison of chorionic gonadotropin expression in human and macaque ( Macaca fascicularis ) trophoblasts
We have designed novel DNA primers that allow us to detect the expression of the subunits of chorionic gonadotropin (CG) from a variety of species of the order Primates. Using these primers, reverse transcriptase‐polymerase chain reaction (RT‐PCR), and standard cloning techniques, we detected the expression of a single gene for the common glycoprotein hormone (GPH) α‐subunit and at least two genes for the CG β‐subunit in trophoblasts of Macaca fa…
Noninstrumented enzyme‐linked immunosorbant assay for detection of early pregnancy in macaques
A practical, noninstrumented enzyme‐linked immunosorbant assay (NELISA) for the measurement of urinary monkey chorionic gonadotropin (mCG) has been developed for the detection of early pregnancy in macaque monkeys for use in both the laboratory and the field. Five rhesus monkeys ( Macaca mulatta ) and six crab‐eating monkeys ( Macaca fascicularis ) were tested for the presence of mCG in urine on gestational days (GDs) 12 to 35. The mCG NELISA det…
Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques
An enzyme-linked immunosorbent assay (ELISA) for human urinary beta follicle-stimulating hormone (FSH) subunit was validated for use in the laboratory macaque (Macaca mulatta and Macaca fasicularis). This ELISA is based on the dissociation of the FSH heterodimer in urine and the subsequent measurement of the beta subunit as a representation of total urinary FSH. This assay was then used to describe the gonadotropin escape following ovarian senesc…
Elisa for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the beta subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has be…
The relationship of serum estradiol and progesterone concentrations to the enzyme immunoassay measurements of urinary estrone conjugates and immunoreactive pregnanediol‐3‐glucuronide in Macaca mulatta
Paired urine and serum samples from four conceptive and six nonconceptive ovarian cycles of seven adult Macaca mullatta were analyzed by radioimmunoassay (RIA) for circulating estradiol (E 2 ) and progesterone (Po), and urinary estrone conjugates (E 1 C) and immunoreactive preganediol‐3‐glucuronide (iPDG) using enzyme immunoassay (EIA). Nonconceptive cycles exhibited a fivefold increase in urinary E 1 C and serum E 2 levels from follicular phase …
No prominent works on this page.
The relationship of serum estradiol and progesterone concentrations to the enzyme immunoassay measurements of urinary estrone conjugates and immunoreactive pregnanediol‐3‐glucuronide in Macaca mulatta
Paired urine and serum samples from four conceptive and six nonconceptive ovarian cycles of seven adult Macaca mullatta were analyzed by radioimmunoassay (RIA) for circulating estradiol (E 2 ) and progesterone (Po), and urinary estrone conjugates (E 1 C) and immunoreactive preganediol‐3‐glucuronide (iPDG) using enzyme immunoassay (EIA). Nonconceptive cycles exhibited a fivefold increase in urinary E 1 C and serum E 2 levels from follicular phase …
Elisa for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
A rapid, sensitive, enzyme-linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well-characterized monoclonal antibody (Mab, 518B7) generated against the beta subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has be…
Application of an enzyme immunoassay for urinary follicle-stimulating hormone to describe the effects of an acute stressor at different stages of the menstrual cycle in female laboratory macaques
An enzyme-linked immunosorbent assay (ELISA) for human urinary beta follicle-stimulating hormone (FSH) subunit was validated for use in the laboratory macaque (Macaca mulatta and Macaca fasicularis). This ELISA is based on the dissociation of the FSH heterodimer in urine and the subsequent measurement of the beta subunit as a representation of total urinary FSH. This assay was then used to describe the gonadotropin escape following ovarian senesc…
Noninstrumented enzyme‐linked immunosorbant assay for detection of early pregnancy in macaques
A practical, noninstrumented enzyme‐linked immunosorbant assay (NELISA) for the measurement of urinary monkey chorionic gonadotropin (mCG) has been developed for the detection of early pregnancy in macaque monkeys for use in both the laboratory and the field. Five rhesus monkeys ( Macaca mulatta ) and six crab‐eating monkeys ( Macaca fascicularis ) were tested for the presence of mCG in urine on gestational days (GDs) 12 to 35. The mCG NELISA det…
Comparison of chorionic gonadotropin expression in human and macaque ( Macaca fascicularis ) trophoblasts
We have designed novel DNA primers that allow us to detect the expression of the subunits of chorionic gonadotropin (CG) from a variety of species of the order Primates. Using these primers, reverse transcriptase‐polymerase chain reaction (RT‐PCR), and standard cloning techniques, we detected the expression of a single gene for the common glycoprotein hormone (GPH) α‐subunit and at least two genes for the CG β‐subunit in trophoblasts of Macaca fa…
Contribution of ovarian steroid production to urinary estrone conjugate concentrations in Macaca mulatta
This study was designed to test the hypothesis that basal estrone conjugate (E 1 C) profiles do not accurately detect ovarian function when ovarian estrogen production is low or absent. We employed surgical removal of active ovaries from laboratory rhesus macaques to simulate an acute decline in ovarian estrogen production. In the first experiment, urine samples collected prior to and following ovariectomy (Ovx) were subjected to high‐performance…
Biology (6 works) · Medicine (6 works) · Hormone (5 works) · Urinary system (5 works) · Urine (5 works) · Endocrinology (4 works) · Hypothalamic control of reproductive hormones (4 works) · Antibody (3 works) · Chemistry (3 works) · Internal Medicine (3 works)