The Effects of Environmental Endocrine Disrupter Bisphenol A on Learning-memory and Synaptic Structure of Adult Mice

The Effects of Environmental Endocrine Disrupter Bisphenol A on Learning-memory and Synaptic Structure of Adult Mice

Dados Bibliográficos

ID19407448
AutoresXingyi Liu (0000-0002-2406-651X), Xiaohong Xu (0000-0001-9970-2173), Qin Zhang (0000-0002-7537-9129), Guangxia ZHANG, Jialin Ji (0000-0001-5611-1904), Fangni DONG, Yanling Yang (0000-0002-8069-2283)
Ano2013
Volume45
Fascículo9
Páginas981-992
Data de publicação2013-12-13
Peer ReviewedSim
Open AccessNão
TipoARTICLE
PeriódicoActa Psychologica Sinica (JOURNAL)
Identificadores do periódicoISSN: 0439-755X
EditoraChina Science Publishing & Media Ltd (PUBLISHER)
DOI10.3724/sp.j.1041.2013.00981
OpenAlexW2315691223
IdiomaEN
Citações recebidas1
Referências citadas1

摘要: 双酚A (bisphenol, BPA) 是一种广泛存在的环境内分泌干扰物, 它可与雌激素受体结合干扰内源性雌激素对中枢神经系统的调控作用。本研究通过将10周龄小鼠灌胃染毒BPA (0.4、4、40 mg/kg/day)3个月, 研究长期BPA暴露对成年小鼠记忆行为和突触可塑性的影响。开场行为测试结果表明, BPA (0.4、4、40 mg/kg/day)增加雄性的站立次数和理毛频率, BPA (4 mg/kg/day)却显著减少雌鼠的站立次数。水迷宫和被动回避行为模型检测显示, BPA主要损伤雄鼠的空间学习记忆和被动回避记忆。通过制备海马CA1区超薄切片后, 电镜观测发现, BPA (0.4、40 mg/kg/day) 暴露降低雄鼠海马CA1区突触数密度, 缩短雄鼠突触前活性带长度, 减小雄鼠突触后致密体(PSD)厚度, 增加雄鼠突触间隙宽度。进一步用Western blot方法检测突触前、后的标志性蛋白Synapsin I和PSD95以及兴奋性氨基酸NMDA受体NR1亚基和AMPA受体GluR1亚基蛋白的表达, 发现BPA暴露致雄鼠Synapsin I、PSD95、NR1蛋白表达水平下调。而BPA对雌鼠的记忆行为、突触形态、突触蛋白和受体蛋白均没有明显作用。以上结果提示长期BPA暴露性别特异性地影响成年小鼠的活动性和探究行为, 损伤雄鼠的学习记忆, 这些作用可能通过下调突触蛋白和NMDA受体的表达而负性影响突触结构可塑性, 最终影响雄鼠的学习记忆功能

Biology · Bisphenol A · Endocrine system · Hormone · Chemistry · Effects and risks of endocrine disrupting chemicals · Medicine · Neuroscience · Psychology · Endocrinology · Internal Medicine