A unified protocol for simultaneous extraction of DNA and proteins from archaeological dental calculus
Bibliographic Data
| ID | 11901083 |
|---|---|
| Authors | Zandra Fagernäs (0000-0003-2667-3556, Max Planck Institute for the Science of Human History), Maite I García-Collado (0000-0003-4324-0197, University of the Basque Country), Jessica Hendy (0000-0002-3718-1058, Max Planck Institute for the Science of Human History), Courtney A Hofman (0000-0002-6808-3370, University of Oklahoma), Camilla Speller (0000-0001-7128-9903, University of British Columbia), Irina M Velsko (0000-0001-9810-9917, Max Planck Institute for the Science of Human History), Irina Velsko, Christina Warinner (0000-0002-4528-5877, Harvard University, corresponding author) |
| Year | 2020 |
| Volume | 118 |
| Pages | 105135-105135 |
| Publication date | 2020-04-24 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Journal of Archaeological Science (JOURNAL) |
| Journal identifiers | ISSN: 0305-4403 • E-ISSN: 1095-9238 |
| Publisher | Elsevier BV (PUBLISHER) |
| DOI | 10.1016/j.jas.2020.105135 |
| OpenAlex | W3020673914 |
| Language | EN |
| Citations received | 8 |
| References cited | 53 |
Archaeological materials are a finite resource, and efforts should be made to minimize destructive analyses. This can be achieved by using protocols combining extraction of several types of biomolecules or microparticles, which decreases the material needed for analyses while maximizing the information yield. Archaeological dental calculus is a source of several different types of biomolecules, as well as microfossils, and can tell us about the human host, microbiome, diet, and even occupational activities. Here, we present a unified protocol allowing for simultaneous extraction of DNA and proteins from a single sample of archaeological dental calculus. We evaluate the protocol on dental calculus from six individuals from a range of time periods and estimated preservation states, and compare it against previously published DNA-only and protein-only protocols. We find that most aspects of downstream analyses are unaltered by the unified protocol, although minor shifts in the recovered proteome can be detected, such as a slight loss of hydrophilic proteins. Total protein recovery depends on both the amount of starting material and choice of extraction protocol, whereas total DNA recovery is significantly reduced using the unified protocol (mean 43%). Nevertheless, total DNA recovery from dental calculus is generally very high, and we found no differences in DNA fragment characteristics or taxonomic profile between the protocols. In conclusion, the unified protocol allows for simultaneous extraction of two complementary lines of biomolecular evidence from archaeological dental calculus without compromising downstream results, thereby minimizing the need for destructive analysis of this finite resource
Ancient DNA · Archaeology · Calculus (dental · Geography · Protocol (science · Archaeology and ancient environmental studies · Computer Science · Dentistry · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Medicine
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| Citations per year | 1.6 |
|---|---|
| Citation span | 2021 - 2026 (6) |
| Highly cited | No |