Presence of Sars-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022

Bibliographic Data

ID14968534
AuthorsCarlos Cimmino (Instituto Nacional de Epidemiología “Dr. Juan H. Jara”, Mar del Plata, Argentina., corresponding author), Leandro Rodrigues Capítulo (0000-0003-2074-7980, Universidad Nacional de La Plata), Andrea Lerman (Instituto Nacional de Epidemiología “Dr. Juan H. Jara”, Mar del Plata, Argentina.), Andrea P Silva (0000-0002-4791-9706), Andrea Silva (0000-0002-0207-6362, Instituto Nacional de Epidemiología “Dr. Juan H. Jara”, Mar del Plata, Argentina.), Gabriela Von Haften (Obras Sanitarias Sociedad de Estado, Mar del Plata, Argentina.), Ana Paula Comino, Luciana Cigoy (Obras Sanitarias Sociedad de Estado, Mar del Plata, Argentina.), Marcelo Scagliola (Obras Sanitarias Sociedad de Estado, Mar del Plata, Argentina.), Verónica Poncet (0000-0001-8155-5478, Instituto Nacional de Epidemiología “Dr. Juan H. Jara”, Mar del Plata, Argentina.), Gonzalo Caló (0000-0001-8858-9291, Instituto de Investigaciones en Biodiversidad y Biotecnología and FIBA, Mar del Plata, Argentina.), Osvaldo UEZ (Instituto Nacional de Epidemiología “Dr. Juan H. Jara”, Mar del Plata, Argentina.), Corina M Berón (0000-0002-0365-3571, Instituto de Investigaciones en Biodiversidad y Biotecnología and FIBA, Mar del Plata, Argentina.)
Year2023
Volume47
Pages1
Publication date2023-06-12
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueRevista Panamericana de Salud Pública (JOURNAL)
Journal identifiersISSN: 1020-4989 • E-ISSN: 1680-5348
PublisherPan American Health Organization (PUBLISHER • US)
DOI10.26633/rpsp.2023.94
PMID37324201
OpenAlexW4380318508
LanguageEN
References cited31

Objectives. To implement and evaluate the use of wastewater sampling for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two coastal districts of Buenos Aires Province, Argentina. Methods. In General Pueyrredon district, 400 mL of wastewater samples were taken with an automatic sampler for 24 hours, while in Pinamar district, 20 L in total (2.2 L at 20-minute intervals) were taken. Samples were collected once a week. The samples were concentrated based on flocculation using polyaluminum chloride. RNA purification and target gene amplification and detection were performed using reverse transcription polymerase chain reaction for clinical diagnosis of human nasopharyngeal swabs. Results. In both districts, the presence of SARS-CoV-2 was detected in wastewater. In General Pueyrredon, SARS-CoV-2 was detected in epidemiological week 28, 2020, which was 20 days before the start of an increase in coronavirus virus disease 2019 (COVID-19) cases in the first wave (epidemiological week 31) and 9 weeks before the maximum number of laboratory-confirmed COVID-19 cases was recorded. In Pinamar district, the virus genome was detected in epidemiological week 51, 2020 but it was not possible to carry out the sampling again until epidemiological week 4, 2022, when viral circulation was again detected. Conclusions. It was possible to detect SARS-CoV-2 virus genome in wastewater, demonstrating the usefulness of the application of wastewater epidemiology for long-term SARS-CoV-2 detection and monitoring

Biology · Coronavirus disease 2019 (COVID-19 · Disease · Gene · Infectious disease (medical specialty · Real-time polymerase chain reaction · Sampling (signal processing · Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 · Veterinary medicine · Wastewater · Biosensors and Analytical Detection · Environmental Science · Medicine · SARS-CoV-2 and COVID-19 Research · SARS-CoV-2 detection and testing · Environmental Engineering · Epidemiology · Internal Medicine · Virology

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