Pollen Monitoring by Optical Microscopy and DNA Metabarcoding
Comparative Study and New Insights
Bibliographic Data
| ID | 15467935 |
|---|---|
| Authors | Mattia Fragola (0000-0003-0384-6821, University of Salento), Augusto Arsieni (Azienda Sanitaria Locale (ASL) Brindisi, 72100 Brindisi, Italy), Nicola Carelli (Agenzia Regionale per la Protezione dell'ambiente ligure), Sabrina Dattoli (Agenzia Regionale per la Protezione dell'ambiente ligure), Sante Maiellaro (ASL Roma), M R Perrone (0000-0001-5319-4716, University of Salento), Salvatore Romano (0000-0003-0856-4989, University of Salento, corresponding author) |
| Year | 2022 |
| Volume | 19 |
| Issue | 5 |
| Pages | 2624-2624 |
| Publication date | 2022-02-24 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph19052624 |
| PMID | 35270312 |
| OpenAlex | W4213437498 |
| Language | EN |
| References cited | 39 |
Environmental samples collected in Brindisi (Italy) by a Hirst-type trap and in Lecce (Italy) by a PM10 sampler were analysed by optical microscopy and DNA-metabarcoding, respectively, to identify airborne pollen and perform an exploratory study, highlighting the benefits and limits of both sampling/detection systems. The Hirst-type trap/optical-microscopy system allowed detecting pollen on average over the full bloom season, since whole pollen grains, whose diameter vary within 10-100 μm, are required for morphological detection with optical microscopy. Conversely, pollen fragments with an aerodynamic diameter ≤10 μm were collected in Lecce by the PM10 sampler. Pollen grains and fragments are spread worldwide by wind/atmospheric turbulences and can age in the atmosphere, but aerial dispersal, aging, and long-range transport of pollen fragments are favoured over those of whole pollen grains because of their smaller size. Twenty-four Streptophyta families were detected in Lecce throughout the sampling year, but only nine out of them were in common with the 21 pollen families identified in Brindisi. Meteorological parameters and advection patterns were rather similar at both study sites, being only 37 km apart in a beeline, but their impact on the sample taxonomic structure was different, likely for the different pollen sampling/detection systems used in the two monitoring areas
Biological dispersal · Biology · Optics · Physics · Pollen · Sampling (signal processing · Allergic Rhinitis and Sensitization · Indoor Air Quality and Microbial Exposure · Plant Pathogens and Fungal Diseases · Ecology
| Citation velocity | historical |
|---|---|
| Highly cited | No |