Characterization of the Bacterial Community Naturally Present on Commercially Grown Basil Leaves
Evaluation of Sample Preparation Prior to Culture-Independent Techniques
Bibliographic Data
| ID | 15474387 |
|---|---|
| Authors | Siele Ceuppens (0009-0001-8628-1987, Ghent University), Stefanie Delbeke (Ghent University), Dieter De Coninck (0000-0002-0685-7553, Ghent University), Jolien Boussemaere (Ghent University), Nico Boon (0000-0002-7734-3103, Ghent University), Mieke Uyttendaele (0000-0002-3134-8929, Ghent University, corresponding author) |
| Year | 2015 |
| Volume | 12 |
| Issue | 8 |
| Pages | 10171-10197 |
| Publication date | 2015-08-21 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph120810171 |
| PMID | 26308033 |
| OpenAlex | W1825746529 |
| Language | EN |
| Citations received | 1 |
| References cited | 63 |
Fresh herbs such as basil constitute an important food commodity worldwide. Basil provides considerable culinary and health benefits, but has also been implicated in foodborne illnesses. The naturally occurring bacterial community on basil leaves is currently unknown, so the epiphytic bacterial community was investigated using the culture-independent techniques denaturing gradient gel electrophoresis (DGGE) and next-generation sequencing (NGS). Sample preparation had a major influence on the results from DGGE and NGS: Novosphingobium was the dominant genus for three different basil batches obtained by maceration of basil leaves, while washing of the leaves yielded lower numbers but more variable dominant bacterial genera including Klebsiella, Pantoea, Flavobacterium, Sphingobacterium and Pseudomonas. During storage of basil, bacterial growth and shifts in the bacterial community were observed with DGGE and NGS. Spoilage was not associated with specific bacterial groups and presumably caused by physiological tissue deterioration and visual defects, rather than by bacterial growth
16S ribosomal RNA · Bacteria · Biology · Flavobacterium · Food spoilage · Microbial population biology · Microbiological culture · Pantoea · Pseudomonas · Temperature gradient gel electrophoresis · Mycorrhizal Fungi and Plant Interactions · Plant Pathogens and Fungal Diseases · Plant-Microbe Interactions and Immunity · Food Science
| Citations per year | 0.09 |
|---|---|
| Citation span | 2015 - 2015 (1) |
| Highly cited | No |