Purificación de la fracción antigénica 27-28 KDA a partir del antígeno metabólico secretado excretado de Fasciola hepatica
Bibliographic Data
| ID | 15534622 |
|---|---|
| Authors | Isidro Antitupa (0000-0002-8684-767X, Instituto Nacional de Salud, corresponding author), William Quispe (0009-0006-0492-8975, Instituto Nacional de Salud), Jhon Mayo (Instituto Nacional de Salud), Fanny Valverde (Instituto Nacional de Salud), Elizabeth Sánchez (0000-0001-8179-1657, Instituto Nacional de Salud) |
| Year | 2014 |
| Volume | 31 |
| Issue | 2 |
| Pages | 288-91 |
| Publication date | 2014-07-02 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Revista Peruana de Medicina Experimental y Salud Pública (JOURNAL) |
| Journal identifiers | ISSN: 1726-4634 • E-ISSN: 1726-4642 |
| Publisher | National Institute of Health of Peru (PUBLISHER • PE) |
| DOI | 10.17843/rpmesp.2014.312.48 |
| PMID | 25123868 |
| OpenAlex | W1626144427 |
| Language | ES |
| Citations received | 1 |
| References cited | 2 |
Antigenic fractions of 27-28 kDa from Fasciola hepatica were purified by size-exclusion chromatography for use in the diagnosis of human fasciolosis. Excretion and secretion antigens were obtained from living adult flukes collected from sheep and cattle liver, and cultured in minimum essential medium. The reactivity of the purified antigen and efficacy were assessed by immunoblot test using four sera with human fascioliasis; four sera with other parasites, and two negative sera. We conclude that the purified antigenic fractions do not cross-react with other parasites by immunoblot. Therefore, purified proteins are considered as potential candidates to be used for the diagnosis of human fascioliasis
Antigen · Biology · Cross reactions · Cross-reactivity · Fasciola · Fasciola hepatica · Fasciolosis · Helminths · Hepatica · Liver fluke · Coccidia and coccidiosis research · Helminth infection and control · Parasites and Host Interactions · Immunology · Molecular Biology
| Citations per year | 0.33 |
|---|---|
| Citation span | 2023 - 2023 (1) |
| Highly cited | No |