Resistoma y genómica comparativa de aislados clínicos de Escherichia coli diarreogénica en Lima, Perú

Bibliographic Data

ID15534872
AuthorsWilli Quino (0000-0002-3693-1531, Instituto Nacional de Salud, corresponding author), Orson Mestanza (0000-0001-7268-0496, Instituto Nacional de Salud), Junior Caro-Castro (0000-0002-8890-6119, Instituto Nacional de Salud), Carmen V Hurtado (0000-0003-3667-6806, Instituto Nacional de Salud), Ronnie G Gavilán (0000-0003-1437-5607, Universidad Privada San Juan Bautista)
Year2020
Volume37
Issue4
Pages705-10
Publication date2020-11-18
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueRevista Peruana de Medicina Experimental y Salud Pública (JOURNAL)
Journal identifiersISSN: 1726-4634 • E-ISSN: 1726-4642
PublisherNational Institute of Health of Peru (PUBLISHER • PE)
DOI10.17843/rpmesp.2020.374.5240
PMID33566911
OpenAlexW3108160211
LanguageES
References cited2

In order to describe the genomic characteristics related to antimicrobial resistance and comparative genomics of diarrheagenic Escherichia coli (DEC), 14 DEC isolates from the strain collection of the Instituto Nacional de Salud (INS) were subjected to genome sequencing. We used bioinformatic procedures to analyze the obtained sequences in order to look for microbial resistance genes and genetic regions related to pathotypes and phylogroups. Several antimicrobial resistance determinants were detected, but the production of beta-lactamases and mutations associated to quinolone resistance were the most relevant. Additionally, we observed isolates of the same pathotype grouped in different phylogroups. The comparative genomics analysis showed a greater number of orthologous genes in isolates from the same pathotype and phylogroup. In conclusion, DEC isolates from Lima, Peru, showed resistance to multiple drugs; likewise, molecular and phylogenetic diversity was observed in several pathotypes and phylogroups

Biology · Humanities · Philosophy · vaccines and immunoinformatics approaches · Molecular Biology