Analysis of the Extracts of Isatis tinctoria by New Analytical Approaches of HPLC, MS and NMR
Bibliographic Data
| ID | 20346802 |
|---|---|
| Authors | Jiawen Zhou (0000-0002-6817-1071, Zhejiang Gongshang University), Fan Qu (0000-0003-1851-1514, Women's Hospital, School of Medicine, Zhejiang University) |
| Year | 2011 |
| Volume | 8 |
| Issue | 5S |
| Pages | 33-45 |
| Publication date | 2011-07-15 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Journals Online (AJOL) (PUBLISHER) |
| DOI | 10.4314/ajtcam.v8i5s.13 |
| PMID | 22754056 |
| PMCID | PMC3252730 |
| OpenAlex | W2020009118 |
| Language | EN |
| Citations received | 1 |
| References cited | 1 |
The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method
Atmospheric-pressure chemical ionization · Chemical ionization · Chromatography · Chromatography detector · Electrospray · Electrospray ionization · High-performance liquid chromatography · Ion · Ionization · Mass spectrometry · Chemistry · Essential Oils and Antimicrobial Activity · Genomics, phytochemicals, and oxidative stress · Pharmacological Effects of Natural Compounds · Organic Chemistry
| Citations per year | 0.07 |
|---|---|
| Citation span | 2011 - 2011 (1) |
| Highly cited | No |