A two-step extraction method to measure fecal steroid hormones in female cynomolgus monkeys (Macaca fascicularis)
Bibliographic Data
| ID | 22898649 |
|---|---|
| Authors | Mie Matsumuro, Tadashi Sankai (0000-0002-6100-1176, National Institute of Infectious Diseases), Fumiaki Cho (National Institute of Infectious Diseases), Yasuhiro Yoshikawa (National Institute of Infectious Diseases), Takashi Yoshida (0000-0003-1119-312X, National Institute of Infectious Diseases, corresponding author) |
| Year | 1999 |
| Volume | 48 |
| Issue | 4 |
| Pages | 291-298 |
| Publication date | 1999-01-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | American Journal of Primatology (JOURNAL) |
| Journal identifiers | ISSN: 0275-2565 • E-ISSN: 1098-2345 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1002/(sici)1098-2345(1999)48:4<291::aid-ajp4>3.0.co;2-6 |
| PMID | 10402037 |
| OpenAlex | W2021450593 |
| Language | EN |
| Citations received | 5 |
| References cited | 18 |
We developed a two-step extraction method for measuring fecal steroid concentrations. In the first step, distilled water was used to extract steroids from fecal samples. In the second step, a mixture of organic solvents (hexane and ether) was used to re-extract water extracts that had been transferred to a glass tube. A portion of the upper layer of the organic solvents was transferred to separate assay-tubes for measurement of estradiol (E2) or progesterone (P), and the organic solvents were evaporated in vacuo. After phosphate-buffered saline was added to each tube, commercially supplied radioimmunoassay (RIA) kits were used to determine the steroids. We demonstrated the advantages and reliability of this method by using it to assay the steroid hormone concentrations in fecal samples and serum samples collected on the same day from female cynomolgus monkeys who showed normal menstrual cycles and from monkeys who had induced hyperfunction of ovarian steroidgenesis. Different fecal samples from each monkey were used to determine the recovery rate of each steroid in water extraction from the fecal samples and the reproductivity of hormone concentrations in the fecal samples. The results demonstrate that this two-step method is simple and effective for measuring fecal steroids for monitoring the reproductive status of cynomolgus monkeys, without having to collect serum samples.
Biology · Chromatography · Distilled water · Extraction (chemistry) · Feces · Hormone · Radioimmunoassay · Steroid · Steroid hormone · Chemistry · Endocrinology · Hypothalamic control of reproductive hormones · Internal Medicine · Medicine · Physiology · Primate Behavior and Ecology · Stress Responses and Cortisol
Monitoring the reproductive status of Japanese monkeys (Macaca fuscata) by measurement of the steroid hormones in fecal samples
Fecal testosterone immunoreactivity as a non-invasive index of functional testosterone dynamics in male Japanese macaques (Macaca fuscata)
Monitoring the reproductive status of female gorillas (Gorilla gorilla gorilla) by measuring the steroid hormones in fecal samples
Assessing Gestation and Fetal Sex in Wild Assamese Macaques Using Urinary Estrogen Analysis
Male-Induced Sociosexual Behavior by Vaginal Secretions in Macaca arctoides
Information content of sexual swellings and fecal steroids in sooty mangabeys (Cercocebus torquatus atys)
Measurement of excreted steroids in Macaca nemestrina
Fecal steroid analysis of ovarian cycles in free‐ranging baboons
Assessment of female reproductive status in captive‐housed Hanuman langurs ( Presbytis entellus ) by measurement of urinary and fecal steroid excretion patterns
Application of fecal steroid techniques to the reproductive endocrinology of female verreaux's Sifaka ( Propithecus verreauxi )
| Citations per year | 0.2 |
|---|---|
| Citation span | 2001 - 2025 (25) |
| Highly cited | No |