Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography

Bibliographic Data

ID23196396
AuthorsElias Antonio Pizarro (0000-0002-7899-5015, University of Tarapacá), Xiaofeng Ma (0009-0008-6683-0927), Pengfei Tu (0000-0002-3848-8174), Waldo Ríos Bordones (University of Tarapacá), Yingjie Chen (0000-0001-6705-3535), Tianyou Zhang, Yun WEI (0009-0002-5085-8446), Yoichiro Ito (0000-0001-9018-295X)
Year2003
Volume992
Issue1-2
Pages193-197
Publication date2003-04-01
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueJournal of Chromatography A (JOURNAL)
Journal identifiersISSN: 0021-9673 • E-ISSN: 1873-3778
PublisherElsevier BV (PUBLISHER)
DOI10.1016/s0021-9673(03)00315-7
OpenAlexW12735475
LanguageEN
Citations received10
References cited5

Two isoflavones, calycosin-7-O-beta-D-glycoside and formononetin-7-O-beta-D-glycoside, were separated from n-butanol extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography in two steps using two different solvent systems composed of ethyl acetate-ethanol-n-butanol-water (30:10:6:50, v/v) and ethyl acetate-ethanol-water (5:1:5, v/v). From 200 mg of crude extract, calycosin-7-O-beta-D-glycoside (12 mg) and formononetin-7-O-beta-D-glycoside (10 mg) were isolated at over 95% purity by HPLC analyses, and their structures were identified by MS, 1H NMR and 13C NMR.

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