STR-genotyping of archaeological human bone
Experimental design to improve reproducibility by optimisation of DNA extraction
Bibliographic Data
| ID | 3778945 |
|---|---|
| Authors | Wera M Schmerer (0000-0003-2467-1362, University of Göttingen), Hummel (0000-0002-3647-8968), Susanne Hummel, Bernd Herrmann (0009-0000-1851-8746) |
| Year | 2000 |
| Volume | 58 |
| Issue | 1 |
| Pages | 29-35 |
| Publication date | 2000-03-28 |
| Open Access | No |
| Type | ARTICLE |
| Venue | Anthropologischer Anzeiger (JOURNAL) |
| Journal identifiers | ISSN: 0003-5548 • E-ISSN: 2363-7099 |
| Publisher | S ch we iz er ba rt (PUBLISHER) |
| DOI | 10.1127/anthranz/58/2000/29 |
| PMID | 10816783 |
| OpenAlex | W2412999359 |
| Language | EN |
| References cited | 1 |
The analysis of degraded DNA with the help of short tandem repeat loci (STRs) is an important source of information both in forensic casework and in the anthropological context. The reproducibility of STR-genotyping of highly degraded or "ancient" DNA can be reduced by the generation of artifacts during PCR amplification. The frequency and amount of these artifacts--allelic dropout and the generation of shadow bands--are related to the quality and quantity of the extracted DNA amplified in a PCR reaction. Therefore, one important strategy to increase the reproducibility of STR-genotyping of samples containing degraded DNA is the optimisation of the DNA extraction.
Allele · Ancient DNA · Biology · Chromatography · Context (archaeology) · DNA · DNA extraction · Gene · Genotype · Genotyping · Microsatellite · Polymerase chain reaction · Population · Reproducibility · STR analysis · Chemistry · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Genetics · Medicine · Molecular Biology Techniques and Applications · Paleontology
| Highly cited | No |
|---|