Staining ground sections of natural dry bone tissue for microscopy
Bibliographic Data
| ID | 4692989 |
|---|---|
| Authors | H H De Boer (0000-0001-8590-0945, Leiden University Medical Center, corresponding author), M J Aarents (Leiden University Medical Center), G J R Maat (Leiden University Medical Center) |
| Year | 2012 |
| Volume | 22 |
| Issue | 4 |
| Pages | 379-386 |
| Publication date | 2012-07-01 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Osteoarchaeology (JOURNAL) |
| Journal identifiers | ISSN: 1047-482X • E-ISSN: 1099-1212 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1002/oa.1208 |
| OpenAlex | W2050893061 |
| Language | EN |
| Citations received | 8 |
| References cited | 20 |
The histological staining of bone tissue is of great value in identifying structural changes in human bone tissue when examined microscopically, and is therefore routinely used by clinical pathologists. However, for archaeological and other types of undecalcified dry bone tissue, histochemical staining is currently not widely used. The purpose of this paper is to describe a new method developed for the staining of undecalcified archaeological bone which may be a valuable addition to the palaeopathological toolkit. Sections were ground by hand and stained with haematoxylin and eosin. The microstructure and architecture of the stained sections become clearly visible without compromising total tissue envisioning. In addition, staining enhances differentiation between taphonomically affected and non-affected bone tissue. This paper accordingly describes a reproducible stepwise method for the production of ground stained sections. An additional troubleshooting paragraph discusses the most often encountered problems and provides solutions. Copyright (c) 2010 John Wiley & Sons, Ltd
Bone tissue · Eosin · H&E stain · Haematoxylin · Pathology · Staining · Dental materials and restorations · Endodontics and Root Canal Treatments · Forensic Anthropology and Bioarchaeology Studies · Medicine · Anatomy
Paleohistology and the study of human remains
Assessing Post-traumatic Time Interval in Human Dry Bone
A quantitative histological comparison of ground human bone preparation techniques
An Approach to the Diagnosis of Craniofacial Fibrous Dysplasia from the 2500‐Year‐Old Remains of a Skull from Ancient China
Technical note
A comparative analysis of microscopic alterations in modern and ancient undecalcified and decalcified dry bones
Manual for the Preparation and Staining of Embedded Natural Dry Bone Tissue Sections for Microscopy
Different Preparation Techniques - Similar Results? On the Quality of Thin-Ground Sections of Archaeological Bone
Microscopical Focal Destruction (Tunnels) in Exhumed Human Bones
Histological analysis of undecalcified thin sections of archeological bone
Manual preparation of ground sections for the microscopy of natural bone tissue
A test of the revised Frost's 'rapid manual method' for the preparation of bone thin sections
Soil pH, Bone Preservation, and Sampling Bias at Mortuary Sites
| Unique citing works | 8 |
|---|---|
| Citations per year | 0,62 |
| Citation span | 2013 - 2018 (6) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 6 |