Manual for the Preparation and Staining of Embedded Natural Dry Bone Tissue Sections for Microscopy
Bibliographic Data
| ID | 4723788 |
|---|---|
| Authors | H H De Boer (0000-0001-8590-0945, Dept. of Anatomy, Barge's Anthrop. Leiden University Medical Centre Leiden The Netherlands, corresponding author), M J Aarents (Dept. of Anatomy, Barge's Anthrop. Leiden University Medical Centre Leiden The Netherlands), G J R Maat (Dept. of Anatomy, Barge's Anthrop. Leiden University Medical Centre Leiden The Netherlands) |
| Year | 2013 |
| Volume | 23 |
| Issue | 1 |
| Pages | 83-93 |
| Publication date | 2013-01-01 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Osteoarchaeology (JOURNAL) |
| Journal identifiers | ISSN: 1047-482X • E-ISSN: 1099-1212 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1002/oa.1242 |
| OpenAlex | W1823655849 |
| Language | EN |
| Citations received | 17 |
| References cited | 34 |
In the last decade, the use of light microscopy has been firmly established for the investigation of exhumated human bone tissue. As a rule, these remains cannot be decalcified; thus, they are most commonly prepared for microscopic analysis as ground thin sections. These ground sections are of great value in diagnosing disease, in estimating age or in assessing taphonomic alteration. As bone is sometimes fragile and can be damaged by the grinding process, the specimen is occasionally supported by an embedding medium.In contrast to the vast amount of research conducted on embedded and unembedded unstained bone material, the use of histological stains on undecalcified dry bone tissue has been long neglected. In this article, a new method for embedding, sawing and grinding dry bone tissue is presented. The produced sections are subsequently stained with haematoxylin. The results show that even ground sections of fragile bone can be made in a quick and easy manner. Staining these sections enhances the envisioning of micro-architecture and taphonomical processes. In addition, the sections stay open for inspection under polarized light. The results were consistent throughout the used bone material. To keep the method as accessible and comprehensive as possible, a step-wise manual is provided. An additional troubleshooting paragraph discusses the most often encountered problems and provides solutions. Copyright (c) 2011 John Wiley & Sons, Ltd
Bone tissue · Composite material · Grinding · Haematoxylin · Microscopy · Microtome · Pathology · Staining · Taphonomy · dental development and anomalies · Forensic Anthropology and Bioarchaeology Studies · Materials Science · Medicine · Pleistocene-Era Hominins and Archaeology · Biomedical Engineering · Geology
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Paleohistology and the study of human remains
Assessing Post-traumatic Time Interval in Human Dry Bone
The origin of bacteria responsible for bioerosion to the internal bone microstructure
An Investigation of Micro-CT Analysis of Bone as a New Diagnostic Method for Paleopathological Cases of Osteomalacia
Precarious adolescence
Four possible cases of osteomalacia
Dry bone histology of bone tumours
A quantitative histological comparison of ground human bone preparation techniques
Scurvy as a factor in the loss of the 1845 Franklin expedition to the Arctic
An Odontoma in Domestic Cattle ( Bos taurus ) From the Early Neolithic Site of Međureč (Serbia)
Immaculate conceptions
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Different Preparation Techniques - Similar Results? On the Quality of Thin-Ground Sections of Archaeological Bone
Severe traumatic lesions in the Late Neolithic cattle from the site of At-Vršac, Serbia
Animal diseases in the southern part of the Carpathian Basin during the medieval period
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A test of the revised Frost's 'rapid manual method' for the preparation of bone thin sections
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| Unique citing works | 17 |
|---|---|
| Citations per year | 1,21 |
| Citation span | 2012 - 2026 (15) |
| Citation velocity | current |
| Highly cited | No |
| Citation types | Neutral: 13 |