Peripheral Blood Lymphocytes Adenosine Triphosphate Availability Among the Residents of the Northern European Russia

Metabolic pathways participating in adenosine triphosphate (ATP) synthesis play an important role in lymphocytes normal development and functioning. Energetic metabolism has an influence on differentiation, proliferation and fate of lymphocytes. The aim of the study is to define ATP availability of peripheral blood lymphocytes and the level of mitochondrial metabolism regulator sirtuin 3 (SIRT3) in healthy northerners. Methods. 106 volunteers, habitants of Arkhangelsk (76 women and 30 men, 21-63 years old) were surveyed. Amounts of immunocompetent cells with receptors (CD3, CD4, CD8, CD10, CD16, CD71, CD23, CD25, HLA DR, CD95) were determined by indirect immunoperoxidase method, ATP concentration in lymphocytes by bioluminescent method, and additionally SIRT3 (23 volunteers) by enzyme immunoassay. Two groups were selected using "k-means" cluster analysis which differed statistically significantly in all studied parameters. Results. It was found that 78 % of the surveyed participants were in the group where ATP concentration was 0,95 (0,487) mcmol/106 cells, in other group with 22 % participants it was 3,71 (1,319) mcmol/106 cells, р

Adenosine diphosphate · Adenosine triphosphate · Antibody · Biology · CD19 · CD23 · CD3 · CD38 · CD8 · IL-2 receptor · Immune system · Platelet · Stem cell · T cell · Biochemical effects in animals · Medicine · Sirtuins and Resveratrol in Medicine · Tryptophan and brain disorders · Biochemistry · Cell Biology · Endocrinology · Immunology · Internal Medicine