Extinct species identification from late middle Pleistocene and earlier Upper Pleistocene bone fragments and tools not recognizable from their osteomorphological study by an enhanced proteomics protocol
Bibliographic Data
| ID | 8353483 |
|---|---|
| Authors | Fabrice Bray (0000-0002-4723-8206, CNRS, UAR 3290 ‐ MSAP University of Lille Lille France, corresponding author), Stéphanie Flament (0000-0002-0135-675X, CNRS, UAR 3290 ‐ MSAP University of Lille Lille France), Grégory Abrams (0000-0002-0616-4335, Espace muséal d'Andenne Sclayn Belgium), Dominique Bonjean (0000-0002-0334-8307, Espace muséal d'Andenne Sclayn Belgium), Christian Rolando (0000-0003-3542-5239, CNRS, UAR 3290 ‐ MSAP University of Lille Lille France), Caroline Tokarski (0000-0001-7296-3330, University of Bordeaux Bordeaux France), Patrick Auguste (0000-0002-8302-6307, Univ. Lille, CNRS, UMR 8198 ‐ Evo‐Eco‐Paléo Unité Evolution Lille France) |
| Year | 2023 |
| Volume | 65 |
| Issue | 1 |
| Pages | 196-212 |
| Publication date | 2023-02-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Archaeometry (JOURNAL) |
| Journal identifiers | ISSN: 0003-813X • E-ISSN: 1475-4754 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1111/arcm.12800 |
| OpenAlex | W4283160055 |
| Language | EN |
| Citations received | 2 |
| References cited | 78 |
Ancient preserved molecules offer the opportunity of gaining a deeper knowledge on their biological past. However, the development of a proteomic workflow remains a challenge. The analysis of fossils must involve a low quantity of material to avoid damaging the samples. In this study an enhanced proteomic protocol was applied to 5‐milligram samples of about 130,000‐year‐old mammalian bones ranging from the end of the Middle Pleistocene up to the earlier Upper Pleistocene, excavated from Scladina Cave (Sclayn, Belgium). Using sequence homology with modern sequences, a biological classification was successfully achieved and the associated taxonomic ranks to each bone were identified consistently with the information gained from osteomorphological studies and palaeoenvironmental and palaeodietary data. Amino acid substitutions on collagens were identified, thus providing new information on extinct species sequences and helping in taxonomy‐based clustering. Considering samples with no osteomorphological information, such as two fragments of bone retouchers, proteomics successfully identified the families providing paleontologists new information on these objects. Combining osteomorphology studies and amino acid variations identified by proteomics, one of the retouchers was potentially identified as belonging to the Ursus spelaeus species
Archaeology · Biology · Cave · Computational biology · Evolutionary biology · Gene · Geography · Identification (biology) · Pleistocene · Proteomics · dental development and anomalies · Ecology · Forensic Anthropology and Bioarchaeology Studies · Genetics · Identification and Quantification in Food · Paleontology
A guide to ancient protein studies
Species identification by analysis of bone collagen using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry
A Simple Method for the Isolation and Purification of Total Lipides From Animal Tissues
Paleobiological Implications of the Isotopic Signatures ( 13 C, 15 N) of Fossil Mammal Collagen in Scladina Cave (Sclayn, Belgium)
Species identification using ZooMS, with reference to the exploitation of animal resources in the medieval town of Odense
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen
Advances in proboscidean taxonomy & classification, anatomy & physiology, and ecology & behavior
DeamiDate 1.0
The effects of demineralisation and sampling point variability on the measurement of glutamine deamidation in type I collagen extracted from bone
Examining collagen preservation through glutamine deamidation at Denisova Cave
Distinguishing between archaeological sheep and goat bones using a single collagen peptide
Species identification of ancient Lithuanian fish remains using collagen fingerprinting
Fish ’n chips
A new Cambrian black pigment used during the late Middle Palaeolithic discovered at Scladina Cave (Andenne, Belgium)
ZooMS identification of bone tools from the North African Later Stone Age
Bone retouchers from Lower Palaeolithic sites
Palaeoenvironmental and Palaeodietary Implications of Isotopic Biogeochemistry of Last Interglacial Neanderthal and Mammal Bones in Scladina Cave (Belgium)
When Neanderthals used cave bear (Ursus spelaeus) remains
Mitogenomics of the Extinct Cave Lion, Panthera spelaea (Goldfuss, 1810), Resolve its Position within the Panthera Cats
Combined osteomorphological, isotopic, aDNA, and ZooMS analyses of sheep and goat remains from Neolithic Ulucak, Turkey
| Citations per year | 0.67 |
|---|---|
| Citation span | 2023 - 2026 (4) |
| Highly cited | No |